A proteome-wide prediction of Aurora-B-specific substrate sites
The predicted phosphorylation sites are bold. ROCK, Rho kinase; PKC, protein kinase C; LOK, lymphocyte-oriented kinase; PAK, p21-activated kinase; GFAP, glial fibrillary acidic protein; DES, desmin; NES, nestin; VIM, vimentin; AURKA, Aurora kinase A; CENPA, centromere protein A; BPTF, bromodomain PHD finger transcription factor; MCAK, mitotic centromere-associated kinesin; INCENP, inner centromere protein.
| Substrate | Phospho.ELM | Site | Peptide | GPS score | Known kinases |
|---|---|---|---|---|---|
| HEC1 | O14777 | 5 | ---MKRSSVSSGGAG | 6.1515 | Aurora-B |
| O14777 | 15 | SGGAGRLSMQELRSQ | 4.8788 | Aurora-B | |
| O14777 | 49 | KLSINKPTSERKVSL | 3.8182 | Aurora-B | |
| O14777 | 55 | PTSERKVSLFGKRTS | 5.3636 | Aurora-B | |
| O14777 | 62 | SLFGKRTSGHGSRNS | 3.2424 | ||
| O14777 | 69 | SGHGSRNSQLGIFSS | 4.303 | Aurora-B | |
| AURKA | O14965 | 288 | APSSRRTTLCGTLDY | 5.8485 | PKA, Aurora-A |
| PPP1R12A | O14974 | 696 | ARQSRRSTQGVTLTD | 5.0606 | ROCK1 |
| Survivin | O15392 | 117 | KNKIAKETNNKKKEF | 4.2424 | Aurora-B |
| VIM | P08670 | 65 | GVYATRSSAVRLRSS | 4.303 | PAK |
| P08670 | 72 | SAVRLRSSVPGVRLL | 4.5758 | PAK, ROCK, Aurora-B | |
| GFAP | P14136 | 7 | -MERRRITSAARRSY | 8.303 | ROCK, Aurora-B |
| P14136 | 13 | ITSAARRSYVSSGEM | 7.5152 | ROCK, PKC, CaMKII, Aurora-B | |
| P14136 | 38 | LGPGTRLSLARMPPP | 4.5455 | ROCK, PKC, CaMKII, Aurora-B | |
| STMN1 | P16949 | 62 | AAEERRKSHEAEVLK | 5.8182 | PKA |
| DES | P17661 | 11 | YSSSQRVSSYRRTFG | 4.7576 | Aurora-B |
| P17661 | 16 | RVSSYRRTFGGAPGF | 5.2727 | ROCK, Aurora-B | |
| P17661 | 59 | VYQVSRTSGGAGGLG | 4.8788 | Aurora-B | |
| LMNB1 | P20700 | 27 | PLSPTRLSRLQEKEE | 3.303 | |
| PSMA3 | P25788 | 242 | AEKYAKESLKEEDES | 3.1818 | CK2 |
| CDC25B | P30305 | 353 | VQNKRRRSVTPPEEQ | 6 | Aurora-A |
| BDKRB2 | P30411 | 373 | SMGTLRTSISVERQI | 3.6364 | GRK-4, PKC |
| NES | P48681 | 767 | ETQQRRRSLGEQDQM | 6.8788 | |
| CENPA | P49450 | 7 | -MGPRRRSRKPEAPR | 6.3636 | Aurora-A, Aurora-B |
| PLK1 | P53350 | 137 | LELCRRRSLLELHKR | 3.2424 | |
| P53350 | 210 | YDGERKKTLCGTPNY | 3.2121 | LOK | |
| H3.1 | P68431 | 10 | TKQTARKSTGGKAPR | 10.1212 | Aurora-A, Aurora-B |
| P68431 | 28 | ATKAARKSAPATGGV | 8.9091 | MAPK, Aurora-B | |
| MDM2 | Q00987 | 157 | SHLVSRPSTSSRRRA | 4.0909 | |
| KIF23 | Q02241 | 911 | NGSRKRRSSTVAPAQ | 6.303 | |
| Q02241 | 912 | GSRKRRSSTVAPAQP | 6.3939 | ||
| RELA | Q04206 | 276 | SMQLRRPSDRELSEP | 3.2121 | RSK-5 |
| BPTF | Q12830 | 77 | PRVHRPRSPILEEKD | 3.0303 | |
| TP53BP1 | Q12888 | 1460 | GAGALRRSDSPEIPF | 3.2424 | |
| CBX3 | Q13185 | 93 | KDGTKRKSLSDSESD | 5.1212 | |
| PIN1 | Q13526 | 16 | PGWEKRMSRSSGRVY | 3.1818 | PKA |
| IFI16 | Q16666 | 132 | GAQKRKKSTKEKAGP | 4.8485 | CK2 |
| RCC1 | Q6NT97 | 11 | KRIAKRRSPPADAIP | 4.4545 | |
| FLJ37981 | Q8N1Q3 | 73 | ETSSLRNSQSENSSL | 5 | |
| MCAK | Q99661 | 95 | IQKQKRRSVNSKIPA | 7.5455 | Aurora-B |
| RACGAP1 | Q9H0H5 | 387 | ETGLYRISGCDRTVK | 3.7879 | Aurora-B |
| INCENP | Q9NQS7 | 897 | KPRYHKRTSSAVWNS | 4.1515 | Aurora-B |
| Q9NQS7 | 898 | PRYHKRTSSAVWNSP | 5.7273 | Aurora-B | |
| Q9NQS7 | 899 | RYHKRTSSAVWNSPP | 3.1818 | Aurora-B | |
| TD-60 | Q9P258 | 43 | RERPERCSSSSGGGS | 4.1515 | |
| BAZ1B | Q9UIG0 | 189 | EDEGRRESINDRARR | 5.7576 | |
| Q9UIG0 | 1342 | KRSSRRQSLELQKCE | 4.6061 | ||
| CDC23 | Q9UJX2 | 582 | NTPTRRVSPLNLSSV | 3.7879 |