Table I

N-Linked glycosite information for each protein identified via the CSC technology

The table lists the protein number (corresponds to supplemental Table S1), the protein name, the number of N-linked glycosylation sites confirmed in the current study, the number of potential N-linked glycosylation sites annotated in Swiss-Prot, whether these N-linked sites listed in Swiss-Prot are potential (i.e. the protein contains the NX(S/T) motif but no experimental evidence is available) or whether there is experimental (exp) evidence, and whether the glycopeptides identified in the current study are consistent with the extracellular domain (i.e. orientation) annotated in Swiss-Prot. Proteins are sorted by the number of potential N-linked glycosylation sites in increasing order. Y, observed glycopeptides map to predicted extracellular domain; N, observed glycopeptides map to predicted intracellular domain; NA, not applicable due to GPI or ECM; Amb, annotation regarding orientation is ambiguous. MHC, major histocompatibility complex.

Protein numberProtein nameNo. identified sitesNo. potential sitesaNo. sites with exp evidenceOrientation consistent?Protein numberProtein nameNo. identified sitesNo. potential sitesaNo. sites with exp evidenceOrientation consistent?
45Solute carrier family 2, facilitated glucose transporter member 1110Y9CD80 antigen560Y
63Sphingosine 1-phosphate receptor 2 (Edg-5)110Y15Ectonucleotide pyrophosphatase/phosphodiesterase family member 1360Y
70Ephrin-A5111NA35Neural cell adhesion molecule 1161Y
87Lipid phosphate phosphohydrolase 2110Amb37Neuroplastin361Y
95Solute carrier family 2, facilitated glucose transporter member 3110Y48Transmembrane protein 16F360Y
99Mast cell antigen 32110Y96Tyrosine-protein kinase receptor UFO160Y
113Sphingosine 1-phosphate receptor 5 or 8110Y102Cleft lip and palate transmembrane protein 1 homolog160Y
117Ephrin-B1110Y114OX-2 membrane glycoprotein160Y
123Aquaporin-1110Y128Calcitonin gene-related peptide type 1160Y
125Myelin protein zero-like protein 1110Y10CD97 antigen370Y
3Adipocyte adhesion molecule120Y25Hematopoietic progenitor cell antigen CD34170Y
18Ephrin type-A receptor 2220Y33N-Acetylated α-linked acidic peptidase 2370Y
20Excitatory amino acid transporter 1220Y56Poliovirus receptor-related protein 1270Y
21Junctional adhesion molecule A120Y73Latrophilin 2170Y
44Sodium/potassium-transporting ATPase subunit β-3120Y86VPS10 domain-containing receptor SorCS2170Y
46Translocon-associated protein α120Y103Cadherin-2170Y
47Transmembrane 4 L6 family member 1120Y112Plexin A1170Y
52Zinc transporter ZIP10220Y119Emilin-1270NA
59Junctional adhesion molecule C120Y14F2 cell surface antigen heavy chain480Y
62Solute carrier family 12 member 2120Y7CD166 antigen480Y
67Neutral amino acid transporter A220Y23Fibronectin480NA
79Transmembrane 9 superfamily member 3120Y34Neogenin280Y
91Glypican-1220NA42Prostaglandin F2 receptor negative regulator280Y
104Trophoblast glycoprotein120Y16Embigin890Y
107Major prion protein120NA17Endothelin-converting enzyme 13100Y
109Tetraspanin-4120Y60Tyrosine-protein kinase-like 72100Y
120Transmembrane protein 87A220Y122Epidermal growth factor receptor1103Y
6Basigin230Y30Integrin αV4110Y
12Choline transporter-like protein 2230Y39β-type platelet-derived growth factor receptor3110Y
19Ephrin type-B receptor 4130Y97Lysosome membrane protein 21110Y
24H-1 class I histocompatability antigen, D-K α chain230Y5Basement membrane-specific heparan sulfate proteoglycan core protein4120NA
32Macrophage mannose receptor 2230Y31Integrin β12120Y
57Protein ITFG3330Y38Oncostatin M-specific receptor subunit β3120Y
80Epithelial membrane protein 1130Amb94Receptor-type tyrosine-protein phosphatase μ1120Y
81Synaptophysin-like protein 1130Y4Aminopeptidase N5130Y
105β-Sarcoglycan130Y27Integrin α36130Y
106LMBR1 domain-containing 1130Y74Lymphocyte antigen 751130Y
108CMP-N-acetylneuraminate-β-galactosamide-α-2,3-sialyltransferase130Y28Integrin α55140Y
110Immunoglobulin superfamily member 3130Y13Chondroitin sulfate proteoglycan 45150Y
115Anthrax toxin receptor 1130Y65Integrin α111160Y
127Cadherin-10130Y88Insulin-like growth factor 1 receptor2160Y
11Cell adhesion molecule 1240Y69Teneurin-32170Y
61CD82 antigen240Y98Leucyl-cystinyl aminopeptidase1170Y
64Tissue factor240Y68Lysosomal membrane glycoprotein 11182Y
77Solute carrier family 12 member 7140Y72Insulin receptor1180Y
78Solute carrier family 12, member 4140Y92Cation-independent mannose 6-phosphate receptor1200Y
83Ephrin type-B receptor 2140Y126Tenascin1201NA
84Thrombomodulin140Y82Probable G-protein-coupled receptor 1261230Y
85Thrombospondin-1140NA41Prolow density lipoprotein receptor-related protein 13510Y
100Kin of IRRE-like protein 1140Y22Fat 1 cadherin30 (30)a0Amb
101Tetraspanin-3140Y76Protein unc-84 homolog B10 (1)a0Amb
118Transmembrane protein 87B140Y14Collectin-1240 (12)a0Y
2Acid sphingomyelinase-like phosphodiesterase 3b250NA40Plexin B260 (15)a0Amb
29Integrin α7150Y49Transmembrane protein 220 (15)a0Amb
36Neuropilin-1250Y116Claudin domain-containing protein 110 (2)a0Amb
50Vascular cell adhesion protein 1 (isoform 1)250Y66MHC H-2K-k protein10 (3)a0Amb
51Voltage-dependent calcium channel subunit α-2/δ-1350Y90Neurotrophin receptor-associated death domain10 (3)a0Amb
54Cadherin-15250Y93Protocadherin 710 (3)a0Amb
55Cation-dependent mannose 6-phosphate receptor150Y26Immunoglobulin superfamily containing leucine-rich repeat region20 (4)a0Amb
58Golgi apparatus protein 1250Y53Zinc transporter ZIP1420 (5)a0N
71Fibroblast growth factor receptor 4150Y89cDNA sequence BC05107010 (5)a0Amb
75P2X purinoceptor 7150Y43Protocadherin γ C310 (8)a0Amb
111Zinc transporter ZIP6150Y121Dolichyl-diphosphooligosaccharide-protein glycosyltransferase subunit STT3B30 (8)a0Amb
124Semaphorin-7A150NA8CD276 antigen21 (4)a0Y
  • a If no N-linked glycosylation sites are predicted in Swiss-Prot or if fewer are predicted than were observed, then the number of predicted N-linked glycosylation sites from EnsembleGly is provided. The first number is from Swiss-Prot; the number in parentheses is from EnsembleGly.