Table I In vivo sumoylated proteins derived after immunoprecipitation from HeLa cells using anti-SUMO1 antibody (see “Experimental Procedures”)

The sequence of the sumoylated peptide and the positions of SUMO acceptor sites as determined by MS and MSMS using ChopNSpice in combination with MASCOT and SEQUEST as search engines are listed. For details, see supplemental Table_S4. Dashes (—) indicate that the corresponding sumoylated peptide or its actual conjugation position could not be identified and thus was not scored by the search engines.

Modification and conjugated proteinSwiss-Prot accession numberSequenceConjugated positionMASCOT scoreSEQUEST XCorr
SUMO1
    Ran GTPase-activating protein 1P46060516LLVHMGLLKSEDKVK530Lys52470.296.10
    Small ubiquitin-related modifier 1P6316517KEGEYIK23Lys1792.875.60
    Small ubiquitin-related modifier 2P619568EGVKTENNDHINLK21Lys1179.505.57
    SUMO-conjugating enzyme UBC9P63279142VEYEKR147Lys14659.845.18
    E3 SUMO-protein ligase RanBP2P49792100IAELLCKNDVTDGRAKYWLER12014.314.34
1408FALVTPKK1415Lys141436.534.22
1715SGFEGMFTKK1724Lys1723106.235.46
2255KNLFR2259Lys225535.552.86
2424FKLQDVADSFKK2434Lys243394.415.12
2507AVVSPPKFVFGSESVK2522Lys251329.652.59
2581NSDIEQSSDSKVK2594Lys259297.716.57
2617AKEK2620Lys261844.606.22
    RanBP2-like and GRIP domain-containing protein 4Q7Z3J3691KAEDIANDALSPEEQEECK709Lys6917.29
    Chromodomain-helicase-DNA-binding protein 8Q9HCK8581YTEDLDIKITDDEEEEEVDVTGPIK609Lys5924.02
    Cytoplasmic dynein 1 heavy chain 1Q142043207KIKETVDQVEELR3219Lys320720.564.21
    Very long-chain-specific acyl-CoA dehydrogenaseP49748633NFKSISK639Lys63546.13
    Bifunctional aminoacyl-tRNA synthetaseP07814314NPIEKNLQMWEEMK327Lys31851.70
SUMO2
    Small ubiquitin-related modifier 2P619568EGVKTENNDHINLK21Lys1137.997.31