The sequence of the sumoylated peptide and the positions of SUMO acceptor sites as determined by MS and MSMS using ChopNSpice in combination with MASCOT and SEQUEST as search engines are listed. For details, see supplemental Table_S4. Dashes (—) indicate that the corresponding sumoylated peptide or its actual conjugation position could not be identified and thus was not scored by the search engines.
Modification and conjugated protein | Swiss-Prot accession number | Sequence | Conjugated position | MASCOT score | SEQUEST XCorr |
---|---|---|---|---|---|
SUMO1 | |||||
Ran GTPase-activating protein 1 | P46060 | 516LLVHMGLLKSEDKVK530 | Lys524 | 70.29 | 6.10 |
Small ubiquitin-related modifier 1 | P63165 | 17KEGEYIK23 | Lys17 | 92.87 | 5.60 |
Small ubiquitin-related modifier 2 | P61956 | 8EGVKTENNDHINLK21 | Lys11 | 79.50 | 5.57 |
SUMO-conjugating enzyme UBC9 | P63279 | 142VEYEKR147 | Lys146 | 59.84 | 5.18 |
E3 SUMO-protein ligase RanBP2 | P49792 | 100IAELLCKNDVTDGRAKYWLER120 | — | 14.31 | 4.34 |
1408FALVTPKK1415 | Lys1414 | 36.53 | 4.22 | ||
1715SGFEGMFTKK1724 | Lys1723 | 106.23 | 5.46 | ||
2255KNLFR2259 | Lys2255 | 35.55 | 2.86 | ||
2424FKLQDVADSFKK2434 | Lys2433 | 94.41 | 5.12 | ||
2507AVVSPPKFVFGSESVK2522 | Lys2513 | 29.65 | 2.59 | ||
2581NSDIEQSSDSKVK2594 | Lys2592 | 97.71 | 6.57 | ||
2617AKEK2620 | Lys2618 | 44.60 | 6.22 | ||
RanBP2-like and GRIP domain-containing protein 4 | Q7Z3J3 | 691KAEDIANDALSPEEQEECK709 | Lys691 | 7.29 | — |
Chromodomain-helicase-DNA-binding protein 8 | Q9HCK8 | 581YTEDLDIKITDDEEEEEVDVTGPIK609 | Lys592 | — | 4.02 |
Cytoplasmic dynein 1 heavy chain 1 | Q14204 | 3207KIKETVDQVEELR3219 | Lys3207 | 20.56 | 4.21 |
Very long-chain-specific acyl-CoA dehydrogenase | P49748 | 633NFKSISK639 | Lys635 | 46.13 | — |
Bifunctional aminoacyl-tRNA synthetase | P07814 | 314NPIEKNLQMWEEMK327 | Lys318 | 51.70 | — |
SUMO2 | |||||
Small ubiquitin-related modifier 2 | P61956 | 8EGVKTENNDHINLK21 | Lys11 | 37.99 | 7.31 |