Table II

Proteins identified in this work as altered in osteoarthritis that have a previously defined mitochondrial localization

ID, identity; TCA, tricarboxylic acid; E, energy; CH, carbohydrate.

RatioProtein IDProtein nameAcc. no.aBiochemical roleLoc.b
Increased in OA
    1.61THIM3-Ketoacyl-CoA thiolase P42765 Lipid metabolismMIT
    1.52KAD4Adenylate kinase isoenzyme 4, mitochondrial P27144 ATP productionMAT
    1.50IMMTMitochondrial inner membrane protein (mitofilin) Q16891 Protein bindingMIM
    1.48ISOC2Isochorismatase domain-containing protein 2 Q96AB3 Pyruvate metabolismMIT
    1.47TRAP1TNF receptor-associated protein 1 (Hsp75) Q12931 Chaperone, Hsp90 familyMIT
    1.41GLSKGlutaminase kidney isoform, mitochondrial precursor O94925 Glutamine catabolismMAT
    1.36NDUV1NADH dehydrogenase (ubiquinone) flavoprotein 1 P49821 Complex I subunit, e to transport chainMIM
    1.32NDUS8NADH dehydrogenase (ubiquinone) iron-sulfur protein 8, mitochondrial precursor O00217 Complex I subunit, e to ubiquinoneMIM
Decreased in OA
    −1.34OPA1Dynamin-like 120-kDa protein, mitochondrial O60313 Mitochondrial fusion, protection from apoptosisMIM, IS
    −1.36VDAC2Voltage-dependent anion-selective channel protein 2 (outer mitochondrial membrane protein porin 2) P45880 Anion transportMOM
    −1.36HIBCH3-Hydroxyisobutyryl-CoA hydrolase, mitochondrial precursor Q6NVY1 Valine catabolismMIT
    −1.39IMMTMitochondrial inner membrane protein (mitofilin) Q16891 Protein bindingMIM
    −1.40ECHATrifunctional enzyme subunit α, mitochondrial (long chain enoyl-CoA hydratase and 3-hydroxyacyl-CoA dehydrogenase) P40939 Fatty acid β oxidationMIT
    −1.52ODPAPyruvate dehydrogenase E1 component α subunit P08559 TCA cycleMAT
    −1.52SODMManganese-superoxide dismutase P04179 Response to ROS stressMAT
    −1.53IDHPIsocitrate dehydrogenase (NADP), mitochondrial precursor P48735 CH metabolism, E productionMIT
    −1.56SODMManganese-superoxide dismutase P04179 Response to ROS stressMAT
    −1.56DHSASuccinate dehydrogenase (ubiquinone) flavoprotein subunit, mitochondrial precursor P31040 Complex II subunit, CH metabolism, TCA cycleMIM
    −1.65SODMManganese-superoxide dismutase P04179 Response to ROS stressMAT
    −1.67IMMTMitochondrial inner membrane protein (mitofilin) Q16891 Protein bindingMIM
    −1.69IDH3AIsocitrate dehydrogenase (NAD) subunit α P50213 CH metabolism, oxidative decarboxylation of pyruvate, TCA cycleMIT
    −1.71ETFAElectron transfer flavoprotein subunit α, mitochondrial precursor P13804 Electron transport chainMAT
    −1.90IDH3AIsocitrate dehydrogenase (NAD) subunit α P50213 CH metabolism, oxidative decarboxylation of pyruvate, TCA cycleMIT
  • a Accession number according to Swiss-Prot/TrEMBL database.

  • b Cellular localization according to sequence and PSORT II program. MIT, mitochondrion; MAT, mitochondrial matrix; MIM, mitochondrial inner membrane; MOM, mitochondrial outer membrane; IS, intermembrane space.