Table I ES proteins of S. japonicum adult worms

aa, amino acids; Seq cov., sequence coverage.

Gi (NCBI) no.AnnotationaaaSMSbSCbSeq cov.SpecNematodedTrematodaeOcf
Ac AB AH ATcL/AE1AE2AEfAF1AF2LF3APASbAS1SS2CS3CS4E
%
Fatty acid binding
    30144605FABP1326676.514270.5+++++6
Chaperone/heat shock protein
    56759038HSP90α271928066826.3
    552242HSP706372776.25913.7++++3
    76155249HSP701371927.63645.3NC++++3
    189502946HSP70-56481522.43311.9SP++++3
    76155211HSP70266860.541941.7++++3
    76156161HSP4514631.73168.8
    76155264HSP97252223.68512.3
    56756997DnaJ homolog A1400225.9348.0
    76154306Chaperonin305155.67411.5NC
    60688010HSP708282.07220.7NC++++3
    76157510CCT724677.1924.1NC
    56753850Endoplasmin79798.723.0SP
    56756959DJ-1 family protein18494.9229.2++2
Structure protein
    1703114Actin-23761929.44733.2NC+++++++++9
    56754341Actin-13601852.24534.7+++++++++9
    56754704Actin, cytoplasm3761347.23223.7+++++++++9
    30995481β-Tubulin443753.791720.5NC+1
    161072α-Tubulin451966.321713.5+1
    29841388α-Tubulin327838.891418.7+1
    56758452CAP124296.725.8NC
Metabolic enzyme
    349802Enolase4341750.43223.7NC+++++++++9
    56757978Pyruvate kinase5611274.83118.5NC+1
    160996GAPDH3381170.52820.1+++++5
    95113705FBA363656.421530.3++++++6
    29841448Phosphoglycerate kinase417615.051413.4+1
    31044498LDH331320.8364.5NC+
    120709GAPDH338196.2457.7+++++5
    29841160Transketolase225207.8513.8+1
    31223056-Phosphofructokinase78198.0933.2++2
    56755469Aldehyde dehydrogenase245141.5735.7NC+++3
    29841244Glutamine synthetase364149.733.3NC
    56758570mMDH341125.6937.0NC++++4
    1255223Triose-phosphate isomerase252178.3539.5NC++2
    56756751Glucosidase, α44387.4225.0NC
    56758850UDP-glucose 4-epimerase35185.2424.0NC
    76156298Phosphoglucose isomerase27469.8222.9NC
    166159344Phosphoglycerate mutase25083.6824.0++2
Detoxification protein
    60279643Thioredoxin peroxidase-2194954.462234.0NC++++4
    1389744GST211834.792024.6+++++++++++11
    117380647SOD-like protein153797.351732.0NC+++++++++9
    38259184Thioredoxin peroxidase-1184536.661218.5NC++++4
    29841216GST218306.78717.0NC+++++++++++11
%
    198385352TGR596147.842.3NC
    3892185PDI480106.2736.9NC+++3
    76155624PDI homolog356189.133.4NC+++3
Muscle contraction
    56752573Titin7611736.93210.8NC
    76156843Titin-like protein36971.524.1NC
Signal transduction
    18950292214-3-3 protein homolog 12542177.74761.4++++4
    5675829414-3-3 protein254822.022127.2++++4
    76155391Creatine kinase242203.15515.7NC
    5675715114-3-3 ϵ251165.2249.2NC++++4
    18950293214-3-3 protein homolog 123188.45213.4NC++++4
Proteolysis
    56756044LAP3981362.93228.1NC+
    2829279Calpain760509.03139.2+1
    56756901Calpain B77373.8425.0+1
    313121ER6048479.3622.5SP
Protein catabolic process
    56753927Ubiquitin C457756.87188.8NC+++3
    32452858Ubiquitin-activating enzyme E565228.1451.9NC
    56756008Ornithine aminotransferase43882.0525.9SP
Transcription or translation
    76154398MF3 protein197455.331028.9
    56759476Transcriptional coactivator213148.9938.0
    29841143Ras-related protein ORAB-1198115.18311.6
    29841162RAB5B214162.9837.0NC
    33348818Elongation factor 1-α46584.6227.1++++4
    60692924RPS21.2 (putative)8961.93216.9NC
Cell cycle
    56755261Cyclophilin 1164487.791121.3++2
Egg protein
    2366425221.7-kDa protein185395.888.6NC+1
    56755421Major egg antigen354240.41514.4++2
    56752663Egg antigen46782.9826.4NC
Immune response
    56754313Annexin359326.8975.0+1
    56757229Annexin B13354312.2279.0+1
    56757962Immunophilin431312.03720.2+1
Muscle protein
    4761226Paramyosin866302.0178.1
    146741272Paramyosin866133.7235.4
    5305387Dynein light chain 189112.74315.7
    29841466Myophilin antigen19081.5627.4NC
Protein binding/folding
    117380649Calreticulin396294.2964.3SP+++3
    29841364LBD3120141.83412.5NC
    29841046Endophilin-B125593.7625.1NC
Protease
    11167Cathepsin B342223.1143.8SP++2
%
Tegument protein
    161127Sj22.619187.4624.7NC+1
    56755910Fasciola/Schistosoma cross-reactive protein156102.97211.5NC
Actin binding
    56755882Actin-binding and severin family group-like protein361144.0145.8
    495668Fimbrin65195.1135.5+1
    76153279Fimbrin391131.0736.1+1
    38683290Actin-binding/filamin-like protein98484.5522.0NC
    1469904JF-251999.5922.9
    76161984Thymosin isoform 29179.3214.3SP+
Nucleotide metabolism
    82697983Adenosine deaminase35281.4325.7
    76156228AsnRS324120.65312.3
    28916483NDK15794.67216.6++2
    56758560MTAP29999.4635.4
Nucleosome assembly
    56752775Protein SET254169.27417.3
Response to stress
    56759388STIP1 protein319232.1555.3
Others
    76155389Creatine kinase240105.2637.5NC
    76154162AFFP257122.0326.2
Unknown protein
    189503024Hypothetical protein236166.02418.6NC
    56758512Hypothetical protein233130.1534.7NC
    29841468Hypothetical protein420101.5724.0
    76157439Hypothetical protein137104.13210.2NC
  • a AFFP, actin filament-fragmenting protein; AsnRS, asparaginyl-tRNA synthetase; CCT7, chaperonin containing TCP1, subunit 7 (η); ER60, ER-luminal cysteine protease ER 60; FABP, FABP variant H or F; FBA, fructose-1,6-bisphosphate aldolase; LAP, leucine aminopeptidase; LBD3, LIM domain-binding protein 3; LDH, lactate dehydrogenase; mMDH, mitochondrial malate dehydrogenase; MTAP, methylthioadenosine phosphorylase; NDK, nucleoside-diphosphate kinase; PDI, protein-disulfide isomerase; PRP19, pre-mRNA processing factor 19; RPS21.2, 40 S ribosomal protein S21 isoform 2; Sj22.6, 22.6-kDa tegument-associated antigen; STIP1, stress-induced phosphoprotein 1; TGR, thioredoxin glutathione reductase.

  • b The relative abundance of ES proteins is represented by summed Mowse peptide score (SMS) or SC. The summed Mowse peptide score is the sum of Mascot scores of all peptides including redundant peptides, not just a simple summed Mascot protein score of distinct peptides.

  • c The ES proteins were predicted to be a classical secretory protein containing an SP or NC secretory protein or not secreted (−) by secretion prediction (Spe) using SecretomeP and SignalP. If the prediction of the representative protein failed to obtain a positive result, then the prediction result of the proteins within the same protein group is shown in italic.

  • d The ES proteome of S. japonicum was compared with that of nematodes including A. caninum (Ac, Ref. 25), B. malayi (B, Ref. 19), Haemonchus contortus (H, Ref. 23), and T. circumcincta (Tc, Ref. 26). A, adult; L, larva.

  • e The ES proteome of S. japonicum was compared with that of Trematoda including E. caproni (E1, Ref. 27; E2, Ref. 34), Echinostoma friedi (Ef, Ref. 28), F. hepatica (F1, Ref. 30; F2, Ref. 31; F3, Ref. 29), P. westermani (P, Ref. 32), S. bovis (Sb, Ref. 33) and S. mansoni (S1, Ref. 34; S2, Ref. 35; S3, Ref. 36; S4, Ref. 35). S, sporocyst; C, cercaria; E, egg.

  • f Oc, occurrence (number of times) across the ES proteomes of different parasites.