Table III LC-MS analysis of SDS-PAGE gel bands from whole worm extracts and ESPs of S. japonicum

aa, amino acids; Seq, sequence; WCL-abd, abundant in WCL extract of adult worms; ES-abd, abundant in ES products; WCL-ag, antigenic in WCL extract of adult worms; ES-ag, antigenic in ES products; SMS, summed Mowse score.

Gel band no.CategoryGi (NCBI) no.AnnotationProtein length (aa)Seq coverageSMSSC
aa%
1WCL-abd1703114Actin-237633.51381.227
1WCL-abd30995481β-Tubulin44311.7239.125
1WCL-abd349802Enolase4342.3225.764
1WCL-abd123599HSP701989.6160.233
1WCL-abd160996GAPDH3385.9181.043
1WCL-abd161072α-Tubulin4513.3179.083
1WCL-abd38683290Actin-binding/filamin-like protein9841.5177.333
1WCL-abd84402Glutathione transferase21910.5168.273
1WCL-abd95113705Fructose-1,6-bisphosphate aldolase3636.6193.263
2WCL-abd2829289HSP7064815.9708.3211
2WCL-abd399939HSP70 homolog6377.1197.053
3WCL-abd3941320Myosin80220.02065.533
3WCL-abd3041711Paramyosin (antigen Sj97)86620.7328.366
4WCL-ag3041711Paramyosin (antigen Sj97)86620.72861.147
5WCL-ag146741272Paramyosin86610.6474.028
5WCL-ag3041711Paramyosin (antigen Sj97)86620.7332.145
5WCL-ag56753909Oncosphere protein Tso22b32812.2322.815
5WCL-ag56759038HSP90α27194.2213.194
5WCL-ag56756044His-tagged cytosolic leucine aminopeptidase3983.8133.362
6WCL-abd76154815Myosin heavy chain97622.6710.1413
6WCL-abd5675829414-3-3 protein2544.7117.712
7ES-ag76154815Myosin heavy chain97622.62194.737
8ES-abd76155249Heat shock protein 7013721.2201.034
8ES-abd76162587Similar to major vault protein11112.6308.214
8ES-abd56756008Ornithine aminotransferase4388.4160.933
8ES-abd56758570Mitochondrial malate dehydrogenase3417.9107.262
8ES-abd60688010Heat shock protein 708217.1137.262
9ES-abd56754704Actin-23769.3773.0716
9ES-abd76154123Lamin b23378.3213.524
9ES-abd76152538Nuclear lamin C protein26610.2142.923
9ES-abd56757081Similar to major vault protein1579.695.882
10ES-abd56758584ATP synthase subunit β, mitochondrial precursor51411.9296.466
10ES-abd56759082Mitochondrial H+-ATPase a subunit20918.2255.385
10ES-abd1174754Tropomyosin-128412.0115.562
11WCL-ag1174754Tropomyosin-128412.02882.645
11WCL-ag1174756Tropomyosin-2 (TMII)28412.7229.884
11WCL-ag18950292214-3-3 protein homolog 12549.1151.542