Table I Identification data

The UniProt accession number (AC column), name, biochemical parameters, and observed peptides are given for the proteins of interest identified. The first two proteins (YM1 and TNF) were identified by MS/MS, and the third protein (lipocalin-2) was identified by peptide mass fingerprinting. Theor., theoretical; Cov., coverage; M-ox, oxidized methionine; C-CAM, carbamidomethylated cysteine; N/A, not applicable; RMS, root mean square.

ACProteinTheor.massTheor.pICov.RMSScoreNo.peptidesSequencesPeptidescore
kDa%
O35744Chitinase-3-likeprotein-3precursor (YM1),Mus musculus(mouse)44.4305.42398272114SPYDIGK30
HLFSVLVK37
TLLAIGGWK28
DYEALNGLK41
HFPLTSTLK41
AFEEESVEK61
QIFIQSVIR42
KAFEEESVEK21
DGYTGENSPLYK51
TGIGAPTISTGPPGK67
SADLNVDSIISYWK69
FGPAPFSAMVSTPQNR108
QYNFDGLNLDWQYPGSR63
IPELSQSLDYIQVMTYDLHDPK62
P06804Tumor necrosisfactor precursor,M. musculus(mouse)25.8795.0140482976VNLLSAVK49
ANALLANGMDLK55
GDQLSAEVNLPK52
GQGCPDYVLLTHTVSR40
DNQLVVPADGLYLVYSQVLFK54
DTPEGAELKPWYEPIYLGGVFQLEK47
ACProteinTheor.massTheor.pICov.RMSScoreNo.peptidesSequencesPeptidemass
kDa%
P11672Lipocalin-2,M. musculus(mouse)22.8618.9649N/A1319 (10 matched masses, 6 unmatchedmasses)ITLYGR722.42
TFVPSSR793.42
ELSPELK815.45
DQDQGCR (C-CAM)878.34
TKELSPELK1044.58
AGQFTLGNMHR1231.58
WYVVGLAGNAVQK1404.74
SLGLKDDNIIFSVPTDQCIDN (C-CAM)2364.17
YPQVQSYNVQVATTDYNQFAMVFFR3016.32
YPQVQSYNVQVATTDYNQFAMVFFR (M-ox)3032.64