Table IV LC-MS/MS profile of proteins expressed in mitochondria

The whole mitochondrial proteins obtained from gel pieces cut out from Fig. 4, lane D were subjected to LC-MS/MS analysis.

Protein nameAcaMolecular massbLoccReleased
Carbamoyl-phosphate synthetase 1P07756166MCa2+
Pyruvate carboxylaseP52873130M
2-Oxoglutarate dehydrogenase E1 componentQ5XI78117M
Ladybird homeobox corepressor 1P84551101N
Dimethylglycine dehydrogenaseQ6334296Mt
Glycerol-3-phosphate acyltransferaseP9756495OM
Aconitate hydrataseQ9ER3486Mt
Trifunctional enzyme subunit αQ6442883M
NADH-ubiquinone oxidoreductase 75-kDa subunitQ66HF180IM
Peroxisomal multifunctional enzyme type 2P9785280Pr
Long-chain fatty acid CoA ligase 1P1816379OM
Peroxisomal bifunctionalP0789679Pr
Carnitine O-palmitoyltransferase 2P1888675IM
Stress-70 proteinP4872174Mt
Chaperone-activity of bc1 complex-likeQ5BJQ073Mt
Succinate dehydrogenase [ubiquinone] flavoprotein subunitQ920L273IM
Very long chain-specific acyl-CoA dehydrogenaseP4595371IM
Electron transfer flavoprotein-ubiquinone oxidoreductaseQ6UPE169IM
Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complexP0846168M
Apoptosis-inducing factor 1Q9JM5367IMS
Carboxylesterase 3NP_579829e62ER
Glutamate dehydrogenase 1P1086062MCa2+
60-kDa heat shock proteinP6303961M
ATP synthase subunit αP1599960IM
CatalaseP0476260Pr
UDP-glucuronosyltransferase 1-1Q6455060Mic
Amine oxidase (flavin-containing) BP1964359OM
Propionyl-CoA carboxylase β chainP0763359M
Seryl-tRNA synthetase, cytoplasmicQ6P79959Cy
Alanine-glyoxylate aminotransferase 2Q6456558Mt
Methylmalonate-semialdehyde dehydrogenaseQ0225358MCa2+
Aldehyde dehydrogenaseP1188457M
Hydroxymethylglutaryl-CoA synthaseP2279157MCa2+
Protein-disulfide isomeraseP0478557ER
4-Aminobutyrate aminotransferaseP5055457M
Amyloid β A4 precursor protein binding family B member 3O3582756?
ATP synthase subunit βP1071956IM
Dihydrolipoyl dehydrogenaseQ6P6R255MCa2+
Fumarate hydrataseP1440855Mt, Cy
Neural Wiskott-Aldrich syndrome proteinO0881655Cy, N
Sulfite oxidaseQ0711655IMSCa2+, val
Ubiquinol-cytochrome-c reductase complex core protein 1Q68FY054IM
Trifunctional enzyme subunit βQ6058752M
Acyl-CoA thioesterase 2O5517150M
2-Oxoisovalerate dehydrogenase subunit αP1196050M
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complexQ0120549Mt
Ornithine aminotransferaseP0418249M
Aspartate aminotransferaseP0050748MCa2+
Kynurenine/α-aminoadipate aminotransferaseQ6460248Mt
Long chain-specific acyl-CoA dehydrogenaseP1565048M
Short/branched chain-specific acyl-CoA dehydrogenaseP7058448M
Ubiquinol-cytochrome-c reductase complex core protein 2P3255148IM
Bile acid CoA:amino acid N-acyltransferaseQ6327647Pr
Isovaleryl-CoA dehydrogenaseP1200747M
Medium chain-specific acyl-CoA dehydrogenaseP0850347MCa2+
Acetyl-CoA acetyltransferaseP1776445MtCa2+
Betaine-homocysteine S-methyltransferaseO0917145Cy
Short-chain specific acyl-CoA dehydrogenaseP1565145MCa2+
α-Methylacyl-CoA racemaseP7047342Mt, PrCa2+
3β-Hydroxysteroid dehydrogenase type 5P2736442ER
3-Ketoacyl-CoA thiolaseP1343742MtCa2+
NADH dehydrogenase (ubiquinone) 1 α subcomplex subunit 10Q561S041M
Ornithine carbamoyltransferaseP0048140MCa2+
Phosphate carrierP1603640IM
d-β-Hydroxybutyrate dehydrogenaseP2914739M
Proto-oncogene tyrosine-protein kinase FERP0976037?
Δ3,52,4-Dienoyl-CoA isomeraseQ6265136Mt, PrCa2+
Malate dehydrogenaseP0463636MCa2+
Sideroflexin-1Q6396536Mt membr
2,4-Dienoyl-CoA reductaseQ6459136MtCa2+
3-Hydroxyisobutyrate dehydrogenaseP2926636MtCa2+
Electron transfer flavoprotein subunit αP1380335MCa2+
Hydroxyacyl-CoA dehydrogenaseQ9WVK735MCa2+
Hydroxymethylglutaryl-CoA lyaseP9751935MCa2+
UricaseP0911835Pr
Tricarboxylate transport proteinP3208934IM
Carnitine/acylcarnitine carrierP9752133IM
Peroxisomal trans-2-enoyl-CoA reductaseQ9WVK333PrCa2+
Prohibitin-2Q5XIH733IM, Cy, N
Thiosulfate sulfurtransferaseP2432933MCa2+
3-Mercaptopyruvate sulfurtransferaseP9753233Mt, CyCa2+
Enoyl-CoA hydrataseP1460432MCa2+
3,2-trans-Enoyl-CoA isomeraseP2396532MCa2+
Voltage-dependent anion channel 1Q9Z2L031OM
Voltage-dependent anion channel 3Q9R1Z031OM
ATP synthase γ chainP3543530IM
ProhibitinP6777930IM
Ubiquinol-cytochrome-c reductase iron-sulfur subunitNP_001008888e30IM
ATP synthase B chainP1951129IM
Electron transfer flavoprotein subunit βQ68FU328MCa2+
ES1 protein homologP5657128Mt
NADH dehydrogenase (ubiquinone) flavoprotein 2P1923428IM
Adenylate kinase isoenzyme 2P2941027IMSCa2+, val
3-Hydroxyacyl-CoA dehydrogenase type-2O7035127Mt
Cytochrome c oxidase subunit 2P0040626IM
Serum amyloid P componentP2368026SCa2+
Aldehyde dehydrogenase family 7 member A1Q6405725?
Glutathione S-transferase Kappa 1P2447325M
Superoxide dismutase (manganese)P0789525M
ATP synthase O subunitQ0664723IM
Peptidyl-prolyl cis-trans isomeraseP2911722MCa2+
Cytochrome c oxidase subunit 4 isoform 1P1088820IM
ATP synthase D chainP3139919IM
ATP synthase δ chainP3543418IM
Succinate dehydrogenase (ubiquinone) iron-sulfur proteinP2191317IM
Cytochrome c oxidase subunit 5AP1124016IM
Cytochrome c oxidase subunit 5BP1207514IM
Ribonuclease UK114P5275914Mt, Cy, N
Cytochrome cP6289812IMSCa2+, val
ATP synthase e chainP294198IM
ATP synthase protein 8P116088IM
  • a Accession number from UniProt.

  • b In kDa.

  • c Location of the individual proteins is shown by the following abbreviations: Cy, cytosol; ER, endoplasmic reticulum; IM, inner membrane; IMS, intermembrane space; M, matrix; Mic, microsome; Mt, mitochondria; Mt membr, mitochondrial membrane; N, nuclei; OM, outer membrane; Pr, peroxisome; S, secretase; ?, unknown.

  • d The proteins revealed to be released from valinomycin-treated or Ca2+-treated mitochondria are shown as val and Ca2+, respectively.

  • e NCBI accession number.