Table I Biochemical analysis of nuclear purity

Nuclear purity was determined on the basis of marker enzyme activities specific for cytosol (alcohol dehydrogenase), microbody (catalase), mitochondria (fumarase hydratase), and plastid (glucose-6-phosphate dehydrogenase). Experiments were done in triplicates. CE, crude extract; N1, nuclear fraction without wash; N2, nuclear fraction after first wash; N, final purified nuclear fraction.

FractionCatalaseAlcohol dehydrogenaseGlucose-6-phosphate dehydrogenaseFumarate hydratase
Activity% of CEActivity% of CEActivity% of CEActivity% of CE
μmol/mg/minμmol/mg/minμmol/mg/minμmol/mg/min
Crude extract28.02 ± 0.581003140 × 10−5 ± 0.00004100158 × 10−4 ± 0.000181006920 × 10−3 ± 0.17100
N15.01 ± 0.02517.88470 × 10−5 ± 0.0000214.9625.9 × 10−4 ± 0.0001516.391090 × 10−3 ± 0.02015.75
N21.08 ± 0.0113.8570 × 10−5 ± 0.000012.224.5 × 10−3 ± 0.000122.848172.50 × 10−3 ± 0.00182.49
N0.035 ± 0.0040.123.3 × 10−5 ± 0.000030.10.36 × 10−4 ± 0.000140.2212.58 × 10−3 ± 0.00190.18