Table I MS/MS identification of collagen I digests

Results are averages from all runs. The top results are in bold. o/n, overnight.

ConditionsaUltrasonicationCOL1 subunitScoreUnique peptidesCoverage
%
o/n digestion (Fig. 1,lane 2)α1397127
α28172712
o/n digestion (Fig. 1, lane 3)α124275
α216755
Surfactant-assisted o/n trypsin digestion (Fig. 1, lane 4)α114714728
α213044231
No trypsin (Fig. 1, lane 5)bvα1000
α2000
UATD (Fig. 1, lane 6)vα110533318
α213804222
UATD then surfactant-assisted o/n digestion (Fig. 1, lane 7)vα127798535
α220266039
  • a n = 7 unless otherwise noted.

  • b n = 4.