Table II Partial list of proteins identified from Matrigel

The number of unique peptides identifying a protein with shared tryptic peptides is in parentheses (homologous identifications). Classic ECM proteins in the top 25 identifications are in bold. SPARC, secreted protein acidic and rich in cysteine.

Protein nameRankAccession no.Unique peptidesProtein scoreBest expect value
Top 25 proteins
    Laminin subunit α11IPI00113726.3150484548.7
    Laminin subunit β12IPI00338785.3113369962.8
    Perlecan (HSPG2)3IPI00515360.8104332637.6
    Laminin subunit γ14IPI00400016.180278955.3
    Nidogen-15IPI00111793.161198258.5
    Laminin subunit α56IPI00116913.32382310.6
    Vimentin7IPI00227299.62981553.6
    Fibronectin 1a8IPI00352163.32884221
    Serum albumin9IPI00131695.32474039.8
    Peroxidasin homolog10IPI00461384.52166421.3
    78-kDa glucose-regulated protein11IPI00319992.12272933.9
    Lamin-B1a12IPI00230394.51544330.1
    ATP synthase subunit β, mitochondrial13IPI00468481.21345938.8
    Fibrinogen β chain14IPI00279079.11543832.4
    Low density lipoprotein receptor-related protein 215IPI00349520.5154734.7
    60-kDa heat shock protein16IPI00308885.61342322
    Myosin-917IPI00123181.4134449.6
    Heat shock 70-kDa protein 9a18IPI00880839.11241621.1
    Prolyl 4-hydroxylase, β polypeptidea19IPI00122815.31132127.1
    SPARC20IPI00126343.11134944.4
    Transitional endoplasmic reticulum ATPase21IPI00622235.51235626.2
    Serotransferrin22IPI00139788.21235620.8
    UDP-glucose ceramide glucosyltransferase-like 123IPI00762897.21235611
    Isoform 2 of spectrin α chain, brain24IPI00753793.2103316.6
    Complement component factor H25IPI00130010.4929012.8
Other ECM proteins
    Laminin β22-1IPI00109612.29(7)2606.5
    Perlecan isoform (HSPG2)3-1IPI00113824.188 (3)281335.5
    Nidogen-25-1IPI00719919.210 (9)31712.6
    Agrn; isoform 2 of agrin36IPI00378698.682477.5
    Collagen α2(IV)43IPI00338452.38/10a234/332a7
    Isoform 2 of collagen α1(XVIII)118IPI00131476.24/1a112/11a4.1
    Collagen α1(IV)120IPI00109588.43/8a92/166a3.1
    Isoform C of fibulin-1170IPI00230432.23745.4
    Laminin subunit α4215IPI00223446.54983.4
    Collagen α1(XV)394IPI00409035.52432.9
    von Willebrand factor A domain-containing protein 1409IPI00331609.71323.9
    Sparcl1; SPARC-like protein 1615IPI00308484.31262
    Collagen α1(I)aIPI00329872.111a239a21.6
    Collagen α2(I)aIPI00222188.43a78a7.7
    Collagen α1(VII)aIPI00134652.32a28a5.1
  • a Identified in collagen-only database search with hydroxylated/oxidized proline as a variable modification.