Table III Partial list of proteins identified from mammary ECM preparation

The number of unique peptides identifying a protein with shared tryptic peptides is in parenthesis (homologous identifications). Classic ECM proteins in the top 25 identifications are in bold. SPARC, secreted protein acidic and rich in cysteine; EGF, epidermal growth factor.

Protein nameRankAccession no.Unique peptidesProtein scoreBest expect value
Top 25 proteins
    Perlecan (HSPG2)1IPI00388323.596312537.5
    Collagen α3(VI)2IPI00565677.274242133.9
    Fibrillin-13IPI00204006.175247132.5
    Collagen α1(XIV)4IPI00360766.453/14a1641/262a36.9
    Fibronectin5IPI00200757.151168432.8
    Filamin-A6IPI00409539.350164631.7
    Laminin β27IPI00212868.145142935.8
    Plectin 68IPI00209000.239117711.8
    AHNAK nucleoprotein9IPI00769072.245147112.3
    Spectrin α chain10IPI00209258.439132523.1
    Periostin, osteoblast-specific factor11IPI00193018.238130052.1
    Tenascin-X12IPI00394165.245129215.4
    Vimentin13IPI00230941.545141769.1
    Serum albumin14IPI00191737.638127468.8
    Laminin γ115IPI00363849.232111733.2
    Lamin-A16IPI00201060.436122945.3
    Myosin-1117IPI00764167.131105920.6
    Nidogen-118IPI00231136.52993028.1
    Laminin α419IPI00361106.42688419.6
    Spectrin β20IPI00555287.22275514.2
    Type II cytoskeletal 521IPI00382153.42574644.1
    Decorin22IPI00199861.12369955.9
    Tubulin β-5 chain23IPI00197579.21757645.9
    Complement inhibitory factor H24IPI00208659.12063823.5
    Lamin-B125IPI00231418.52066542.8
Other ECM proteins
    Perlecan (HSPG2) 284-kDa protein1-1IPI00778528.156 (7)185432.3
    Nidogen-218-1IPI00372786.623 (22)74224.9
    Osteoglycin26IPI00362931.11955841.9
    Collagen α1(VI)27IPI00371853.319/5a626/122a25
    Laminin β128IPI00365542.32368220.4
    Laminin α2 chain30IPI00361301.3175198.1
    Prolargin31IPI00190287.11649234.2
    Collagen α2(VI)36IPI00372839.314/3a490/63a20.6
    Lumican46IPI00206403.11442337
    Biglycan52IPI00191090.11134934.7
    Collagen α1(I)57IPI00188909.28/52a259/1891a4.7
    Collagen α1(XVIII)61IPI00214859.56/2a186/30a5.9
    Collagen α2(I)64IPI00188921.16/40a179/1473a5.5
    Collagen α1(XV)68IPI00364868.36/2a193/43a5.4
    Collagen α2(IV)82IPI00365380.36/2a182/44a6.3
    Fibulin-586IPI00326179.3513914.5
    Laminin α591IPI00190577.451622.5
    Collagen α1(VII)102IPI00767686.24/1a127/13a2.3
    Bone marrow proteoglycan114IPI00206023.1411122.9
    Agrin121IPI00562438.141054
    Papilin, proteoglycan-like sulfated glycoprotein144IPI00367768.341036.1
    Collagen α1(IV)154IPI00362887.32/1a80/19a2.2
    Sulfated glycoprotein 1240IPI00195160.12483.6
    Versican core protein261IPI00190776.12491.2
    von Willebrand factor291IPI00210224.51350.6
    SPARC295IPI00189424.21344.7
    Laminin α1319IPI00363534.44681.9
    von Willebrand factor A domain-containing protein 1337IPI00471665.11324.8
    Similar to collagen α3(VI)380IPI00370868.31270.8
    EGF-containing fibulin-like extracellular matrix protein 1388IPI00365221.41261.8
    Collagen α1(III)aIPI00366944.235a1304a46
    Collagen α1(II)aIPI00394380.45a143a14.6
    Collagen α1(V)aIPI00365380.32a77a8.2
    Collagen α2(V)aIPI00366945.32a68a5
  • a Identified in collagen-only database search with hydroxylated/oxidized proline as a variable modification.