Table I Lectins and antibodies used

Fuc, fucose; LacNAc, N-acetyllactosamine; N/A, not applicable.

NameAbbreviationPrimary specificitySourceCatalog number
Lectins
    Concanavalin AConATerminal αMan, especially bi/triantennary chains to ManVector LaboratoriesB-1005
    Lens culinarisagglutininLCAFucα1,6GlcNAc and αManVector LaboratoriesB-1045
    Phaseolus vulgaris agglutinin-LPHALGalβ1,4GlcNAcβ1,6Man, tetra-antennary complex oligosaccharidesVector LaboratoriesB-1115
    Ulex europaeus agglutininUEATerminal or subterminal Fucα1,2GalVector LaboratoriesB-1065
    Aleuria aurantia lectinAALTerminal αFucVector LaboratoriesB-1395
    Sambucus nigra lectinSNASialic acid attached to terminal galactose in α2,6 (major) and α2,3 (minor)Vector LaboratoriesB-1305
    Peanut agglutininPNATerminal Galβ1,3GalNAcα-Thr/Ser (TF antigen), terminal βGalVector LaboratoriesB-1075
    JacalinJacalinGalβ1,3GalNAcα-Thr/Ser (TF), GalNAcα-Thr/Ser (Tn)Vector LaboratoriesB-1155
    Bauhinia purpurea lectinBPLTerminal β1,3 or β1,4Gal > terminal GalNAcVector LaboratoriesB-1285
    Griffonia simplicifolialectin IGSL-IαGalNAc, GalNAcα-Thr/Ser, and αGalVector LaboratoriesB-1105
    Ricinus communis agglutininRCA120Terminal βGal in lactosamineVector LaboratoriesB-1085
    Sophora japonica agglutininSJAβGalNAc and βGalVector LaboratoriesB-1135
    Vicia villosa lectinVVLTerminal α- or β-linked GalNAcVector LaboratoriesB-1235
    Soybean agglutininSBATerminal α- or β-linked GalNAcVector LaboratoriesB-1015
    Dolichos biflorus agglutininDBAα-Linked GalNAcVector LaboratoriesB-1035
    Lycopersicon esculentum lectinLEL(GlcNAc) and poly(LacNAc) (2–3 consecutive)Vector LaboratoriesB-1175
    Solanum tuberosum lectinSTLGlcNAcβ1,4GlcNAc > lactosamineVector LaboratoriesB-1165
    Wheat germ agglutininWGAGlcNAcβ1,4GlcNAc or lactosamine > sialic acidVector LaboratoriesB-1025
    Griffonia simplicifolia lectin IIGSL-IIα- or β-linked N-acetylglucosamine (GlcNAc)Vector LaboratoriesB-1215
    Erythrina cristagallilectinECLGalβ1,4GlcNAc (lactosamine)Vector LaboratoriesB-1145
NameClone IDTargetSourceCatalog number
Capture antibodies
    Anti-MUC1CA 15-3MUC1US BiologicalC0050-23
    Anti-MUC1SM3MUC1Abcamab22711
    Anti-MUC1VU-11D1MUC1Biogenesis6378-0155
    Anti-MUC15E5MUC1University of NebraskaN/A
    Anti-MUC5ACCLH2MUC5ACChemiconMAB2011
    Anti-MUC5AC45M1MUC5ACBiogenesis1695-0128
    Anti-MUC16CA 125MUC16US BiologicalC0050-01
    Anti-MUC16x325MUC16Abcamab10033
    Anti-CEACAM1GM8G5CEACAM1GenovacGM-0501
    Anti-CEACAM16D305CEACAM1US BiologicalC2589-53A
    Anti-CEACAM52Q397CEACAM5US BiologicalC1299-94
    Anti-CEACAM56D308CEACAM5US BiologicalC2589-76A
    Anti-CEACAM69A6CEACAM6Abcamab26286
    Anti-CEACAM7BAC2CEACAM7Abcamab26281
    Anti-CEACAM76D310CEACAM7US BiologicalC2589-81A
    Anti-pan-CEACAM1,3,4,5,6D14HD11CEACAM1,3,5,6GenovacGM-0505
    Anti-CA 19-91B.844Sialyl-Lewis AUS BiologicalC0075-07
Detection antibodies
    Anti-MUC5ac45M1MUC5ACBiogenesis1695-0128
    Anti-CEACAM56D308CEACAM 5US BiologicalsC2589-76A
    Anti-MUC1VU-11D1MUC1Biogenesis6378-0155
    Anti-pan-CEACAM14HD11CEACAM1,3,4,5,6GenovacGM-0505
    Anti-MUC16CA 125MUC16US BiologicalC0050-01
    Anti-MUC1CA 15-3MUC1US BiologicalC0050-23
    CA 19-91B.844Sialyl-Lewis AUS BiologicalC0075-07
    Anti-sialyl-TnN/ASialyl-Tn (Neu5Acα2,6GalNAcα-Ser/Thr)University of NebraskaN/A