Table II Putative transcription factor binding sites in HDM2-P2 promoter analyzed by the MatInspector program

The DNA sequence of the HDM2-P2 promoter (Chr. 12, contig NC_000012, 67488540–67489062, 523 bp) was analyzed by MatInspector using MatInspector library version 7.1 with an optimized matrix threshold of 0.80.

Family/MatrixaOptimized matrix similarity thresholdbPositioncStranddMatrix similarityeSequence
AP-4
    V$AP4R/V$AP4.010.85−40 ∼ −24(−)0.939agcccCAGCTGgagaca
CTCF
    V$CTCF/V$CTCF.010.80−305 ∼ −281(+)0.820ctctcgcggcggtgGGGGtgggggt
    V$CTCF/V$CTCF.010.80−247 ∼ −223(+)0.815ggtcacgggggccgGGGGctgcggg
NFATC2
    V$NFAT/V$NFAT.010.95−118 ∼ −100(−)0.965agaGGAAaagctgagtcaa
SP1
    V$SP1F/V$SP1.010.88−327 ∼ −313(+)0.911aggaGGGCgggattt
    V$SP1F/V$SP1.010.88−243 ∼ −229(+)0.896acggGGGCcgggggc
    V$SP1F/V$SP1.010.88−152 ∼ −138(+)0.911gtctGGGCgggattg
    V$SP1F/V$SP1.020.85−407 ∼ −393(+)0.851cgggGCGCggggcgc
  • a Matrix name of transcription factor in MatInspector library version 7.1.

  • b Similarity threshold between identified DNA sequence and the matrix of transcription factor binding sequence.

  • c Position of identified DNA sequence from transcriptional start site.

  • d Orientation of identified DNA sequence.

  • e Similarity between the identified DNA sequence and the matrix of transcription factor binding sequence.