Table I UIS assays used for the detection 13 proteins of the E. coli tricarboxylic acid cycle and the individual computed redundancy of each SRM transition

N/A indicates that no MS/MS scan was triggered during the UIS LC/MS/MS analysis preventing independent confirmation for the detection of these peptides. ¦ indicates a fragmentation site. MOWSE, MOlecular Weight SEarch.

UniProtKB/Swiss-Prot entry(gene)Protein namePeptideUIS|2Peptide confirmed by MS/MS (MOWSE score)Computational SRM transition redundancy
b, yAll
Q1Q3a, Q3bQ3aQ3bQ3aQ3b
ACON2_ECOLI (acnB)Aconitate hydratase 2DLV¦H¦AI¦PLYAIK676.90704.43 (y6), 1025.61 (y9)Yes (37)15273260139
676.90888.55 (y8), 1025.61 (y9)12273263139
CISY_ECOLI (gltA)Citrate synthaseAMGIP¦SSMFTVI¦FAMAR915.45595.30 (y5), 1360.67 (y12)Yes (75)22958425277
DHSA_ECOLI (sdhA)Succinate dehydrogenase flavoprotein subunitLP¦GI¦LE¦LSR499.30617.36 (y5), 787.47 (y7)Yes (41)2315535980
499.30375.23 (y3), 787.47 (y7)3695547580
DHSB_ECOLI (sdhB)Succinate dehydrogenase iron-sulfur subunitL¦D¦G¦LSD¦AF¦SVFR663.83726.39 (y6), 1213.58 (y11)Yes (55)103109234169
663.83508.29 (y4), 1098.56 (y10)21880414127
663.83508.29 (y4), 1041.53 (y9)21866414111
DLDH_ECOLI (lpd)Dihydrolipoyl dehydrogenaseGISY¦ETA¦TFPWAASGR857.411063.53 (y10), 1293.62 (y12)Yes (65)12263242144
FUMA_ECOLI (fumA)Fumarate hydratase class I, aerobicV¦A¦P¦EALTLLAR577.35886.53 (y8), 983.59 (y9)Yes (33)5370118179
577.35886.53 (y8), 1054.62 (y10)5392118124
IDH_ECOLI (icd)Isocitrate dehydrogenase (NADP)GP¦L¦T¦T¦PVGGGIR562.82655.37 (y7), 857.48 (y9)Yes (56)16887332174
562.82655.39 (y7), 970.57 (y10)1684533298
562.82756.43 (y8), 970.57 (y10)3404542598
MDH_ECOLI (mdh)Malate dehydrogenaseVA¦V¦L¦G¦AA¦GGI¦GQA¦LALLLK868.04926.60 (y9), 1352.82 (y15)Yes (63)13263253142
868.04926.60 (y9), 1564.98 (y17)13241253152
868.041153.73 (y12), 1295.80 (y14)11760280163
868.041153.73 (y12), 1564.98 (y17)11741280152
868.04670.48 (y6), 1295.80 (y14)26860575163
868.04670.48 (y6), 1465.91 (y16)26848575108
MQO_ECOLI (mqo)Malate:quinone oxidoreductaseVV¦L¦FGPFAT¦FSTK707.39482.26 (y4), 1102.55 (y10)Yes (56)23857419124
707.39482.26 (y4), 1215.64 (y11)23842419138
ODO1_ECOLI (sucA)2-Oxoglutarate dehydrogenase E1 componentVATL¦EDATEMV¦NLYR862.93565.31 (y4), 1340.61 (y11)Yes (78)25464466140
SUCC_ECOLI (sucC)Succinyl-CoA synthetase β chainAV¦LVNI¦F¦GGIVR629.38648.38 (y6), 1087.67 (y10)Yes (34)14145258149
629.38501.32 (y5), 1087.67 (y10)25845400149
ACON1_ECOLI (acnA)Aconitate hydratase 1V¦LLE¦NLLR485.30515.33 (y4), 870.54 (y7)N/A1465226591
SUCD_ECOLI (sucD)Succinyl-CoA ligase (ADP-forming) subunit αS¦GTLTYE¦AVK534.78317.22 (y3), 981.52 (y9)N/A3043940795