Table I Putative Trx1 transnitrosylation target proteins and their S-nitrosylation sites (C)
Accession no. and protein namem/zzLocalization of nitrosylation siteScoreRefs.
Chaperones
    gi|306890, chaperonin (HSP60)986.982237CEFQDAYVLLSEK250a3410, 11, 65
    gi|5729877, heat shock protein 70 protein 8658.362602VCNPIITK60950
    gi|13543666, peptidylprolyl isomerase A (cyclophilin A)985.47256IIPGFMCQGGDFTR6964
809.912155KITIADCGQLE16546
Metabolic proteins
    gi|4503571, enolase 11,003.002344VNQIGSVTESIQACK348a395, 10, 65
    gi|31645, glyceraldehyde-3-phosphate dehydrogenase951.482235VPTANVSVVDLTCR248a1065, 10, 13, 65–73
1,097.522146IISNASCTTNCLAPLAK162a83
    gi|4557032, lactate dehydrogenase B810.392159VIGSGCNLDSAR17052
    gi|4505763, phosphoglycerate kinase 11,070.572107ACANPAAGSVILLENLR12355
    gi|35505, pyruvate kinase955.753343AEGSDVANAVLDGADCIMLSGETAK367a3710
865.92244NTGIICTIGPASR56a42
    gi|37267, transketolase968.433115QAFTDVATGSLGQGLGAACGMAYTGK14036
Structural proteins
    gi|4503745, filamin 11,018.843444CSYQPTMEGVHTVHVTFAGVPIPR467a4773
1,045.5231247LQVEPAVDTSGVQCYGPGIEGQGVFR1272a71
    gi|32015, α-tubulin1,041.133280AYHEQLSVAEITNACFEPANQMVK303a355, 10, 11, 13, 66, 73
787.362339SIQFVDWCPTGFK351a51
    gi|57209813, tubulin, β718.802280NMMAACDPR288a3510, 11, 13, 66, 74
    gi|37852, vimentin931.452322QVQSLTCEVDALK334a4875
Redox
    gi| 4505591, peroxiredoxin 1926.463169HGEVCPAGWKPGSDTIKPDVQK190a6210, 71, 73
    gi|5453549, peroxiredoxin 4925.433241HGEVCPAGWKPGSETIIPDPAGK26363
1,072.833140SINTEVVACSVDSQFTHLAWINTPR16467
Ribosomal proteins
    gi|7765076, S3 ribosomal protein803.52295GLCAIAQAESLR106a3810, 13
    gi|4506605, ribosomal protein L231,171.13216ISLGLPVGAVINCADNTGAK3539
    gi|34335134, SEC13-like 1 isoform b920.793217DVAWAPSIGLPTSTIASCSQDGR23983
640.80228LATCSSDR3545
    gi|4759160, small nuclear ribonucleoprotein929.1039VLHEAEGHIVTCETNTGEVYR29100
    gi|31455238, HNRPA2B1 protein682.82235LTDCVVMR4237
Translation regulators
    gi|4503483, eukaryotic translation elongation factor 2983.452581ETVSEESNVLCLSK594a605, 9, 13
    gi|4503471, eukaryotic translation elongation factor 1α1,122.883396SGDAAIVDMVPGKPMCVESFSDYPPLGR423a715, 9, 65, 73
1,131.603220DGNASGTTLLEALDCILPPTRPTDKPLR247a48
Others
    gi|339723, ADP/ATP translocase616.822120GLGDCLVK127a3410, 76
    gi|56967118, annexin A21,075.002100GLGTDEDSLIEIICSR115a369, 71
    gi|2521981, α2-HS-glycoprotein612.94399CDSSPDSAEDVRK11138
    gi|741376, cathepsin B1,098.032129GQDHCGIESEVVAGIPR14586
    gi|5453854, poly(rC)-binding protein 1830.38247INISEGNCPER57a4010, 65, 73
    gi|188556, migration-inhibitory factor708.40279LLCGLLAER8742
  • a Peptides containing S-nitrosylation sites that have also been previously reported by others. The spectra for the identification of S-nitrosylation sites are shown in supplemental Fig. S10.