Table I Anguimorpha lizard mandibular venom gland mRNA sequences recovered in this study that are homologous to previously characterized Toxicofera toxins

GC-A, guanylyl cyclase A.

Toxin typeSpeciesGenBank accession no.Bioactivity
CRiSPAllEU790958, GU441462, GU441463, GU441464, GU441465, GU441466, GU441467, GU441468, GU441469, GU441470, GU441471, GU441472, GU441473Blockage of ryanodine receptors and potassium channels, producing lethargy, paralysis, and hypothermia (5761)
HelokinestatinC. warreni, G. infernalisGU441510, GU441511Bradykinin inhibition (22, 24); bioactivity determined for new variants from anguid venoms in this study (Fig. 2D).
HyaluronidaseG. infernalis, H. suspectumEU790961, GU441476Venom spreading factor (42)
KallikreinAllEU790962, GU441477, GU441478, GU441479, GU441480, GU441481, GU441482, GU441483, GU441484, GU441485, GU441486, GU441487, GU441488, GU441489, GU441490, GU441491, GU441492, GU441493, GU441494, GU441495, GU441496, GU441497, GU441498, GU441499, GU441500, GU441501, GU441502, GU441503, GU441504, HM437246Release of bradykinin from kinogen (34, 6265); a derivative form also cleaves fibrinogen (66)
LectinO. apodus, V. indicusGU441505, GU441506Snake venom forms have been shown to have various modes of action upon platelet aggregation (67, 68)
Natriuretic peptideC. warreni, G. infernalis, H. suspectum, V. glauerti, V. scalarisEU790965, GU441507, GU441508, GU441509, GU441510, GU441511Hypotension induction leading to loss of consciousness; mediated through the binding of GC-A, resulting in the relaxation of cardiac smooth muscle (4, 16, 20); structure-function studies in this study revealed residues crucial for bioactivity (Fig. 2, B and C)
Nerve growth factorH. suspectum cinctumEU790966Uncharacterized
PLA2C. warreni, H. suspectum, V. gilleni, V. glauerti, V. scalaris, V. tristisEU790967, EU790968, GU441524, GU441525, GU441526, GU441527, GU441528,Inhibition of epinephrine-induced platelet aggregation (4, 69)
VeficolinV. komodoensisHM641898Uncharacterized