Table I

Selected proteins, accession numbers, protein class, and number of optimized peptides detected by LC-MRM-MS (a) and LC-TPM-MS (b)

ProteinSwiss-Prot accession no.Protein classaPeptides predictedAssay optimizationSuccessfully detected as endogenous
a. LC-MRM
    Jak1P52332K22223
    Stat1P42225TF661
    Stat3P42227TF13131
    LifrP42703R333
    Akt1P31750K15135
    P85AP26450K31303
    Total peptides908716
b. LC-TPM
    Pou5f1P20263TF661
    Sox2P48432TF651
    Klf4Q60793TF110
    c-MycP01108TF330
    NanogQ80Z64TF110
    Sall4Q8BX22TF761
    Total peptides24223
  • a TF, transcription factor; R, receptor; K, kinase.