Table I Parameters of magnetic bead handler protocol for biotin-PE immunoassay
StepPlate contentVolumeMixing parameters
TimeTemperatureSpeed
μl
1) TransferMagnetic bead array50
2) Analyte captureSample10030 minRT750 rpm
3) Wash + transferWash buffer10020 sRTSlow
4) Wash + transferWash buffer10020 sRTSlow
5) Bead release to assay plateAssay buffer10020 sRTSlow