Table III Ambient analyte theory: repetitive measurement of biotin-PE from same sample

Shown are the results for five sequentially performed analyses of biotin-PE from the same sample from the same well. The data from the first measurement were used for a five-parametric logistic fit. Using the parameters of this fit, the raw assay data were transformed to analyte concentrations. After five assays, an analyte decrease of 20% was observed in total, suggesting a captured amount of less than 5% per assay. AU, arbitrary units.

Biotin-PEFirst measurementSecond measurementThird measurementFourth measurementFifth measurement
Mean%CVMean%CVMean%CVMean%CVMean%CV
pmAUAUAUAUAU
Blank8881688813813
1822823801723702
531523091293127112533
20113921158310710101519813
803466235801336613211231311
3006181161911599605926258951
pm%CVpm%CVpm%CVpm%CVpm%CV
11213111312
55251514143
20192203180171173
80782831751692671
30031213141276026522601