Table I Protein targets, their respective proteotypic peptides, and performance of individual and multiplexed SISCAPA assays

Recovery is expressed as a percentage. S.D. refers to one standard deviation of the mean. NA, not applicable.

DescriptionPeptideIndividual assayMultiplex assay
RecoveryS.D.RecoveryS.D.
%%
CalumeninSFDQLTPEESK51.03.055.53.3
Disulfide isomeraseVEGFPTIYFAPSGDK84.07.167.03.1
Fibulin-2IGPAPAFAGDTISLTITK13.23.425.81.5
Hypoxia up-regulatedLYQPEYQEVSTEEQR75.037.963.46.9
LegumainDYTGEDVTPENFLAVLR58.59.162.04.2
L-plastinYTLNILEDIGGGQK22.018.351.215.1
OsteopontinGDSLAYGLR76.410.866.05.9
Plectin-8AGTLSITEFADMLSGNAGGFR21.91.821.910.2
Tumor protein D52LGISSLQEFK7.00.98.60.6
Ewing sarcomaGDATVSYEDPPTAK0.00.0NANA
EzrinSQEQLAAELAEYTAK0.10.0NANA
FibrinogenYLQEIYNSNNQK0.30.1NANA
K-caseinGEKNDIVYDEQR0.50.3NANA
NucleobindinAATADLEQYDR0.10.0NANA
Tenascin CVPGDQTSTTIR0.10.0NANA