Table II Variability (reported as %CV) for multiplexed SISCAPA assay performed on 3 separate days in 10 μl of plasma

Peptide concentration (in pg/ml) refers to the spiked peptide level. Protein concentration (in ng/ml) represents the amount of protein that corresponds to a given spike level of the proteotypic peptide, assuming 100% recovery (e.g. complete trypsin digestion). The total %CV values are calculated using all measurements; n refers to the number of independent replicates.

AnalytePeptide concentrationProtein concentration%CV
Day 1Day 2Day 3Totaln
ng/mlng/ml
Calumenin, SFDQLTPEESK0.041.213.124.322.023.49
0.25.917.125.65.916.09
1.029.74.18.614.09.99
5.11480.88.711.611.38
25.674110.14.28.310.79
1283,7066.41.50.715.39
Disulfide isomerase A4, VEGFPTIYFAPSGDK0.311.512.310.414.315.59
1.357.63.57.919.613.89
6.52881.93.814.18.59
32.51,4391.61.39.85.09
1637,1973.72.33.03.89
Fibulin-2, IGPAPAFAGDTISLTITK0.221.112.977.828.839.99
1.21055.824.413.117.19
6.152710.128.817.920.89
30.32,63615.21.96.710.09
15113,18212.45.93.37.89
Hypoxia up-regulated, LYQPEYQEVSTEEQR0.13.637.03.752.255.89
0.317.817.739.318.636.59
1.589.08.112.613.114.99
7.64459.711.05.613.48
38.02,22418.84.08.113.69
19011,12112.72.52.47.59
Legumain, DYTGEDVTPENFLAVLR0.37.914.49.020.316.49
1.639.513.117.914.414.89
7.81974.64.211.98.39
38.89870.74.09.05.49
1944,9378.80.30.94.99
L-plastin, YTLNILEDIGGGQK0.052.14.214.215.516.09
0.210.433.016.914.824.09
1.252.023.334.816.833.59
6.126030.415.711.818.19
30.41,30024.32.617.326.09
1526,50041.21.37.124.39
Osteopontin, GDSLAYGLR0.031.915.931.26.525.99
0.29.69.410.013.311.99
0.848.01.311.016.111.49
3.82404.535.310.924.29
19.01,2002.41.510.637.29
95.16,0004.10.52.131.29
Plectin, AGTLSITEFADMLSGNAGGFR0.716674.321.455.568.99
3.482836.216.719.427.09
16.94,13969.126.920.352.59
84.620,69355.216.223.936.39
423103,46639.515.719.325.29
Tumor protein D52, LGISSLQEFK0.23.222.918.015.022.59
0.916.024.145.819.031.19
4.580.216.722.32.915.09
22.440127.61.09.115.09
1122,00631.11.03.215.69