Table III Limits of detection and quantitation for multiplexed assay performed on 10 μl of plasma

For each peptide, “fmol” corresponds to the amount in the sample, “pm” is the molar concentration, peptide concentrations are reported in pg/ml, and protein concentrations (in ng/ml) represent the amount of protein that corresponds to a given spike level of the proteotypic peptide, assuming 100% recovery (e.g. complete trypsin digestion).

DescriptionPeptideProtein molecular weightLODLOQ
fmolpmPeptide (pg/ml)Protein (ng/ml)fmolpmPeptide (pg/ml)Protein (ng/ml)
CalumeninSFDQLTPEESK37,0640.32532.10.90.76684.52.4
Disulfide isomeraseVEGFPTIYFAPSGDK71,9730.32946.42.10.54878.23.5
Fibulin-2IGPAPAFAGDTISLTITK131,8180.32944.63.90.769103.79.0
Hypoxia up-regulatedLYQPEYQEVSTEEQR111,2090.5531005.91.3133251.914.8
LegumainDYTGEDVTPENFLAVLR49,3730.6641243.11.5150289.97.4
L-plastinYTLNILEDIGGGQK65,0000.21624.61.10.44365.62.8
OsteopontinGDSLAYGLR60,0000.1109.20.60.32726.11.6
Plectin-8AGTLSITEFADMLSGNAGGFR517,3282.01962071015.2520550.4269
Tumor protein D52LGISSLQEFK20,0593.73744197.510.41,0381,16320.8