Table IV Limits of detection and quantitation for multiplexed assay measured directly in plasma with no peptide enrichment

Peptide concentrations are reported in ng/ml, and protein concentrations (in μg/ml) represent the amount of protein that corresponds to a given level of the proteotypic peptide, assuming 100% recovery (e.g. complete trypsin digestion). The column “SISCAPA improvement” reports the difference in LOD determined by direct measurement (no enrichment) and enrichment from 10 μl of plasma (i.e. ratio of enriched LOD/direct LOD).

DescriptionPeptideProtein molecular weightLODLOQSISCAPA improvement
PeptideProteinPeptideProtein
ng/mlμg/mlng/mlμg/ml
CalumeninSFDQLTPEESK37,06420.10.667.11.9626
Disulfide isomeraseVEGFPTIYFAPSGDK71,9731757.758325.83,769
Fibulin-2IGPAPAFAGDTISLTITK131,81890.97.930326.42,039
Hypoxia up-regulatedLYQPEYQEVSTEEQR111,2091327.844325.91,315
LegumainDYTGEDVTPENFLAVLR49,3733218.21,07227.32,597
L-plastinYTLNILEDIGGGQK65,00023610.178933.79,608
OsteopontinGDSLAYGLR60,0003.60.212.10.8391
Plectin-8AGTLSITEFADMLSGNAGGFR517,32897.223.832479.3470
Tumor protein D52LGISSLQEFK20,0591642.95489.8391