Table V Limits of detection and quantitation for multiplexed assay performed on 1 ml of plasma

For each peptide, “fmol” corresponds to the amount in the sample, “pm” is the molar concentration, peptide concentrations are reported in pg/ml, and protein concentrations (in ng/ml) represent the amount of protein that corresponds to a given spike level of the proteotypic peptide, assuming 100% recovery (e.g. complete trypsin digestion).

DescriptionPeptideProtein molecular weightLODLOQ
fmolpmPeptide (pg/ml)Protein (pg/ml)fmolpmPeptide (pg/ml)Protein (pg/ml)
CalumeninSFDQLTPEESK37,0640.60.60.822.21.31.31.647.6
Disulfide isomeraseVEGFPTIYFAPSGDK71,9730.40.40.731.50.90.91.566.4
Fibulin-2IGPAPAFAGDTISLTITK131,8180.30.30.543.70.90.91.3116.8
Hypoxia up-regulatedLYQPEYQEVSTEEQR111,2090.20.20.318.70.40.40.844.3
LegumainDYTGEDVTPENFLAVLR49,3730.20.20.38.60.50.50.922.2
L-plastinYTLNILEDIGGGQK65,0000.20.20.311.50.40.40.624.6
OsteopontinGDSLAYGLR60,0000.10.10.13.60.10.10.17.2
Plectin-8AGTLSITEFADMLSGNAGGFR517,3282.02.04.21,0263.83.88.11,973
Tumor protein D52LGISSLQEFK20,059242427479595966.31,186