Table VI Variability (reported as %CV) for multiplexed SISCAPA assay performed on 1 ml of plasma

Peptide concentration (in pg/ml) refers to the spiked peptide level. Protein concentration (in pg/ml) represents the amount of protein that corresponds to a given spike level of the proteotypic peptide, assuming 100% recovery (e.g. complete trypsin digestion). The total %CV values are calculated using all measurements; all measurements are performed in duplicate.

AnalytePeptide concentrationProtein concentration%CVn
pg/mlpg/ml
Calumenin, SFDQLTPEESK2.059.318.52
10.22976.62
51.21,48314.32
2567,41312.52
1,28037,0641.12
Disulfide isomerase A4, VEGFPTIYFAPSGDK0.523.04.92
2.611514.22
13.057610.12
65.12,8793.92
32514,39516.42
1,62771,9734.92
Fibulin-2, IGPAPAFAGDTISLTITK2.421121.12
12.11,05510.32
60.55,27313.12
30326,3643.42
1,513131,8183.92
Hypoxia up-regulated, LYQPEYQEVSTEEQR3.017834.22
15.28902.32
76.04,4488.92
38022,2428.52
1,899111,2094.22
Legumain, DYTGEDVTPENFLAVLR3.179.016.72
15.53950.22
77.61,9750.32
3889,8759.12
1,93949,3736.22
L-plastin, YTLNILEDIGGGQK2.41041.02
12.252011.92
60.82,6006.12
30413,00013.32
1,52065,0002.72
Osteopontin, GDSLAYGLR0.319.20.52
1.596.03.22
7.64807.02
38.02,4002.22
19012,0006.82
95160,0000.52
Plectin, AGTLSITEFADMLSGNAGGFR3.482840.92
16.94,13969.72
84.620,69346.92
423103,46667.42
2,115517,32858.42
Tumor protein D52, LGISSLQEFK9.016010.82
44.880232.22
2244,01221.12
1,12120,0592.32