Table I Representative proteins detected from deroofed PG+/− cells
Genelog(e)a%/%bUnique peptidesTotal peptidesMolecular massIdentifiercLocationdCellular rolese
kDa
Plec1−147036/45133168517IPI00400209PMAnchors intermediate filaments to desmosomes or hemidesmosomes
Fat1−946.225/4183104507IPI00623114TMCadherin, up-regulated in migration, necessary for efficient wound healing
Lama3−488.416/224662331IPI00125058SecMajor component of basement membranes; mediates the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components
Lamb3−381.431/393451129IPI00117093SecMajor component of basement membranes; mediates the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components
ITGB4−376.929/413751195IPI00313479TMReceptor for laminins, heterodimerizes with ITGA6, participates in invasive carcinomas
Ctnna1−334.339/532838100IPI00112963PMAssociates with the cytoplasmic domain of a variety of cadherins for cell adhesion
Fn1−311.417/272838272IPI00113539SecInvolved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape
Lamc2−298.626/362838130IPI00117115SecMajor component of basement membranes; mediates the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components
Ctnnd1−19526/421926107IPI00751207PMMay associate with and regulate the cell adhesion properties of both C- and E-cadherins
ITGA6−189.920/311821120IPI00227969TMReceptor for laminin in epithelial cells, plays a critical structural role in the hemidesmosome
Htra−188.741/65202951.2IPI00128040SecSerine protease that regulate the availability of IGFs by cleaving IGF-binding proteins
Tnc−156.411/161518222IPI00420656SecExtracellular matrix protein, may play a role in supporting the growth of epithelial tumors
Jup−134.523/33131681.7IPI00229475PMComponent of desmosomes and intermediate junctions, structure and function of submembranous plaques
Angptl2−122.529/37121657.1IPI00126864SecInduces sprouting in endothelial cells through an autocrine and paracrine action
Col17a1−107.28.6/161010148IPI00284644SecIntegrity of hemidesmosome and the attachment of basal keratinocytes to the underlying basement membrane
ITGB1−529.1/186688.2IPI00132474TMRecognizes the extracellular protein sequence RGD in a wide array of ligands
Timp3−1712/213324.2IPI00110370SecIrreversibly inactivates metalloproteases such as collagenase in response to tissue remodeling
  • a X! Tandem measure of how likely the match occurred by chance.

  • b Actual percent coverage vs. percent coverage when corrected for peptides unlikely to be detected by MS with trypsin.

  • c IPI, International Protein Index.

  • d PM, peripheral membrane; TM, transmembrane; Sec, secreted.

  • e Description taken from UniProt. IGF, insulin-like growth factor.