Table I Experimental design for 2D DIGE proteome profiling

Four biological replicate samples for each group (C_1W, control sacrificed at 1 week after vehicle injection; D_1W, 1 week after the confirmation of STZ-induced diabetes; C_2M, 2 months after vehicle injection; and D_2M, 2 months after the confirmation of STZ-induced diabetes) were used and labeled with Cy3 or Cy5. Each gel contained the pooled standard (mixture of equal aliquots of each sample in all experimental groups) and two other subject samples. Thus, the 16 samples were analyzed in triplicate by running eight gels. (For detailed descriptions, refer to “Materials and Methods.”)

GelCy3Cy5Cy2
1C1_1WD1_1WPooled internal standard sample (C1–4_1W + D1–4_1W + C1–4_2 M + D1–4_2M)
2D2_1WC2_1WPooled internal standard sample (C1–4_1W + D1–4_1W + C1–4_2 M + D1–4_2M)
3C3_1wD3_1WPooled internal standard sample (C1–4_1W + D1–4_1W + C1–4_2 M + D1–4_2M)
4D4_1WC4_1WPooled internal standard sample (C1–4_1W + D1–4_1W + C1–4_2 M + D1–4_2M)
5C1_2MD1_2MPooled internal standard sample (C1–4_1W + D1–4_1W + C1–4_2 M + D1–4_2M)
6D2_2MC2_2MPooled internal standard sample (C1–4_1W + D1–4_1W + C1–4_2 M + D1–4_2M)
7C3_2MD3_2MPooled internal standard sample (C1–4_1W + D1–4_1W + C1–4_2 M + D1–4_2M)
8D4_2MC4_2MPooled internal standard sample (C1–4_1W + D1–4_1W + C1–4_2 M + D1–4_2M)