Table I Overview of aberrantly glycosylated human apolipoprotein C-III peptides identified in this study

Urinary peptides from S. mansoni-infected and non-infected individuals were separated using strong cation exchange chromatography. Fractions from urine of the infected individuals containing aberrantly glycosylated peptides from apoC-III were analyzed using LC-ion trap MS/MS, and the glycan composition and peptide backbone were assigned based on the fragment ions. Corresponding fractions from non-infected individuals were analyzed similarly. Sample numbers correspond to MS numbers assigned at the beginning of the study. The previously described glycan composition of the three human apoC-III glycoforms is also shown. y8* refers to the peptide fragment PEVRPTSA. Double charged ions are indicated as [M+2H]. n.a., not applicable; pep, peptide moiety.

Samplesm/z of aberrant apoC-III glycopeptidesFragment ionsPeptide sequenceGlycan composition
Human apoC-III
    ApoC-III0n.a.H1N1a
    ApoC-III1n.a.H1N1S1a
    ApoC-III2n.a.H1N1S2a
Non-infected urines
    10966Not detected
    10967Not detected
    10968Not detected
    10410Not detected
Infected urines
    10411939.3 [M + 3H]3+350.1 (N1F1); 366.1 (H1N1); 512.2 (H1N1F1); 569.2 (H1N2); 715.4 (H1N2F1); 693.7 (pep; [M + 2H]); 795.2 (pep-N1; [M + 2H]); 977.5 (pep-H1N2; [M + 2H]); 1079.0 (pep-H1N3; [M + 2H]); 1160.0 (pep-H2N3; [M + 2H]); 1233.0 (pep-H2N3F1; [M + 2H]); 1261.6 (pep-H2N4; [M + 2H]); 1306.1 (pep-H2N3F2; [M + 2H]); 1334.0 (pep-H2N4F; [M + 2H])WDLDPEVRPTSAH2N4F2
995.8 [M + 3H]3+350.1 (N1F1); 366.1 (H1N1); 512.2 (H1N1F1); 715.4 (H1N2F1); 778.2 (pep; [M + 2H]); 879.8 (pep-N1; [M + 2H]); 1062.5 (pep-H1N2; [M + 2H]); 1135.4 (pep-H1N2F1; [M + 2H]);1163.8 (pep-H1N3; [M + 2H]); 1237.0 (pep-H1N3F1; [M + 2H]); 1245.0 (pep-H2N3; [M + 2H]); 1318.0 (pep-H2N3F1; [M + 2H]); 1347.0 (pep-H2N4; [M + 2H]); 1391.0 (pep-H2N3F2; [M + 2H]); 1420.5 (pep-H2N4F1; [M + 2H])WDLDPEVRPTSAVAH2N4F2
1019.7 [M + 3H]3+350.1 (N1F1); 366.1 (H1N1); 512.1 (H1N1F1); 569.2 (H1N2); 715.4 (H1N2F1); 814.1 (pep; [M + 2H]); 915.7 (pep-N1; [M + 2H]); 1098.5 (pep-H1N2; [M + 2H]); 1171.5 (pep-H1N2F1; [M + 2H]); 1200.0 (pep-H1N3; [M + 2H]); 1273.0 (pep-H1N3F1; [M + 2H]); 1281.1 (pep-H2N3; [M + 2H]); 1354.1 (pep-H2N3F1; [M + 2H]); 1427.2 (pep-H2N3F2; [M + 2H]); 1626.8 (pep); 1830.7 (pep-N); 1992.8 (pep-H1N1); 2138.8 (pep-H1N1F1); 2342.8 (pep-H1N2F2)WDLDPEVRPTSAVAAH3N3F2
    104121044.9 [M + 3H]3+350.1 (N1F1); 366.1 (H1N1); 512.2 (H1N1F1); 715.4 (H1N2F1); 861.3 (H1N2F2); 778.2 (pep; [M + 2H]); 879.8 (pep-N1; [M + 2H]); 1062.9 (pep-H1N2; [M + 2H]); 1136.0 (pep-H1N2F1; [M + 2H]); 1209.0 (pep-H1N2F2; [M + 2H]); 1217.0 (pep-H2N2F1; [M + 2H]); 1237.0 (pep-H1N3F1; [M + 2H]); 1245.0 (pep-H2N3; [M + 2H]); 1310.6 (pep-H1N3F2; [M + 2H]); 1318.0 (pep-H2N3F1; [M + 2H]); 1391.0 (pep-H2N3F2; [M + 2H]); 1464.7 (pep-H2N3F3; [M + 2H])WDLDPEVRPTSAVAH2N4F3
995.8 [M + 3H]3+350.1 (N1F1); 366.1 (H1N1); 512.2 (H1N1F1); 715.4 (H1N2F1)WDLDPEVRPTSAVAH2N4F2
    10413871.5 [M + 3H]3+350.1 (N1F1); 366.1 (H1N1); 512.2 (H1N1F1); 977.5 (pep-H1N2; [M + 2H]); 1050.5 (pep-H1N2F1; [M + 2H]); 1124.0 (pep-H1N2F2; [M + 2H]); 1131.9 (pep-H2N2F1; [M + 2H]); 1159.5 (pep-H2N3; [M + 2H]); 1205.5 (pep-H2N2F2; [M + 2H]); 1233.0 (pep-H2N3F1; [M + 2H])WDLDPEVRPTSAH2N4F3
987.8 [M + 3H]3+350.1 (N1F1); 366.1 (H1N1); 512.2 (H1N1F1); 715.4 (H1N2F1); 693.7 (pep; [M + 2H]); 795.2 (pep-N1; [M + 2H]); 977.5 (pep-H1N2; [M + 2H]); 1050.0 (pep-H1N2F1; [M + 2H]); 1124.1(pep-H1N2F2; [M + 2H]); 1160.0 (pep-H2N3; [M + 2H]); 1233.0 (pep-H2N3F1; [M + 2H]); 1261.5 (pep-H2N4; [M + 2H]); 1306.1 (pep-H2N3F2; [M + 2H]); 1335.6 (pep-H2N4F1; [M + 2H]); 1379.6 (pep-H2N3F3; [M + 2H]); 1408.1 (pep-H2N4F2; [M + 2H])WDLDPEVRPTSAH2N4F3
    104131068.2 [M + 3H]3+350.1 (N1F1); 366.1 (H1N1); 512.2 (H1N1F1); 569.2 (H1N2); 658.2 (H1N1F2); 715.3 (H1N2F1); 861.3 (H1N2F2); 814.0 (pep; [M + 2H]); 915.7 (pep-N1; [M + 2H]); 1098.4 (pep-H1N2; [M + 2H]); 1171.5 (pep-H1N2F1; [M + 2H]); 1244.6 (pep-H1N2F2; [M + 2H]); 1273.0 (pep-H1N3F1; [M + 2H]); 1281.2 (pep-H2N3; [M + 2H]); 1346.1 (pep-H1N3F2; [M + 2H]); 1354.1 (pep-H2N3F1; [M + 2H]); 1418.7 (pep-H1N3F3; [M + 2H]); 1427.4 (pep-H2N3F2; [M + 2H]); 1456.1 (pep-H2N4F1; [M + 2H]); 1500.1 (pep-H2N3F3; [M + 2H]); 1529.0 (pep-H2N4F2; [M + 2H])WDLDPEVRPTSAVAAH2N4F3
1214.8 [M + 3H]3+350.1 (N1F1); 366.1 (H1N1); 512.2 (H1N1F1); 658 (H1N1F2); 715.4 (H1N2F1); 861.3 (H1N2F2); 1062.9 (pep-H1N2; [M + 2H]); 1136.0 (pep-H1N2F1; [M + 2H]); 1165.0 (pep-H1N3; [M + 2H]); 1245.0 (pep-H2N3; [M + 2H]); 1318.0 (pep-H2N3F1; [M + 2H]); 1391.0 (pep-H2N3F2; [M + 2H]); 1419.7 (pep-H2N4F1; [M + 2H]); 1493.7 (pep-H2N4F2; [M + 2H]); 1567.2 (pep-H2N4F3; [M + 2H]); 1574.5 (pep-H3N4F2; [M + 2H]); 1647.6 (pep-H3N4F3; [M + 2H]); 1721.1 (pep-H3N4F4; [M + 2H])WDLDPEVRPTSAVAH3N5F4
    22824802.8 [M + 3H]3+350.1 (N1F1); 366.0 (H1N1); 512.0 (H1N1F1); 876.2 (pep-H1N1; [M + 2H]); 948.7 (pep-H1N1F1; [M + 2H]); 1021.7 (pep-H1N1F2; [M + 2H]);1058.7 (pep-H2N2; [M + 2H]); 1131.7 (pep-H2N2F1; [M + 2H])WDLDPEVRPTSAH2N2F2
1204.2 [M + 2H]2+350.1 (N1F1); 366.0 (H1N1); 512.0 (H1N1F1); 875.7 (pep-H1N1; [M + 2H]); 948.8 (pep-H1N1F1; [M + 2H]); 1022.2 (pep-H1N1F2; [M + 2H]);1058.7 (pep-H2N2; [M + 2H]); 1131.5 (pep-H2N2F1; [M + 2H]); 1385.5 (pep); 1588.6 (pep-N); 1750.7 (pep-H1N1); 1896.7 (pep-H1N1F1); 2042.7 (pep-H1N1F2)WDLDPEVRPTSAH2N2F2
851.5 [M + 3H]3+350.1 (N1F1); 366.0 (H1N1); 512.0 (H1N1F1); 658.1 (H1N1F2); 875.7 (pep-H1N1; [M + 2H]); 948.7 (pep-H1N1F1; [M + 2H]); 1021.7 (pep-H1N1F2; [M + 2H]);1058.6 (pep-H2N2; [M + 2H]); 1131.6 (pep-H2N2F1; [M + 2H]); 1204.7 (pep-H2N2F2)WDLDPEVRPTSAH2N2F3
1214.9 [M + H + Na]2+388.1 (H1N1); 534.1 (H1N1F1); 552.1 (WDLD + Na)+; 856.5 (y8*); 1059.7 (y8*-N); 1367.7 (y8*-H1N1F1); 1570 (y8*-H1N2F1); 1732.8 (y8*-H2N2F1); 1878.8 (y8*-H2N2F2)WDLDPEVRPTSAH2N2F2
1288.2 [M + H + Na]2+388.0 (H1N1); 534.1 (H1N1F1); 552.1 (WDLD + Na); 680.2 (H1N1F2); 856.5 (y8*); 1059.5 (y8*-N); 1367.7 y8*-H1N1F1); 1716.7 (y8*-H1N2F2); 1732.7 (y8*-H2N2F1); 1878.9 (y8*-H2N2F2); 2024.9 (y8*-H2N2F3)WDLDPEVRPTSAH2N2F3
    228281040.8 [M + 3H]3+No fragmentationWDLDPEVRPTSAVAAH4N2F3
    228301040.8 [M + 3H]3+350.1 (N1F1); 366.1 (H1N1); 528.2 (H2N1); 674.2 (H2N1F1); 820.3 (H2N2F2); 814.1 (pep; [M + 2H]); 915.7 (pep-N1; [M + 2H]); 1069.6 (pep-H1N1F; [M + 2H]); 1077.8 (pep-H2N1; [M + 2H]); 1150.9 (pep-H2N1F1; [M + 2H]); 1179.5 (pep-N2H2; [M + 2H]); 1224.1 (pep-H2N1F2; [M + 2H]); 1252.2 (pep-H2N2F2; [M + 2H]); 1260.6 (pep-N2H3; [M + 2H]); 1326.0 (pep-H2N2F2; [M + 2H]); 1341.6 (pep-N2H4; [M + 2H]); 1345.1 (pep-H3N2F1; [M + 2H]); 1407.0 (pep-H3N2F2; [M + 2H]); 1414.0 (pep-N2H4F1; [M + 2H]); 1480.6 (pep- H3N2F3; [M + 2H]); 1487.6 (pep-H4N2F2 [M + 2H]); 1560.6 (pep-H4N2F3 [M + 2H])WDLDPEVRPTSAVAAH4N2F3
  • a See Refs. 22–25.