Table II Predicted secretion pathways of proteins identified in conditioned media from 23 cancer cell lines
Cell lineNo. of identified proteinsPercentage of predicted secreted proteins
Classical secretionaNonclassical secretionbMembrane proteincOthersd
NPC-TW022552351433759.9
NPC-TW042843041646356.6
NPC-BM12923241749656.1
Colo2052954303456357.4
SW4802132501737756.0
SW6202294681672749.5
SK-Hep-12692931637660.6
HepG22672061229062.6
Hep-3B3835733982454.7
OEC-M13334222955958.4
SCC-42094872778348.0
U12803051846856.3
U4364687441,04751.1
MCF-72193902368648.0
MDA-MB-435S2535891792148.3
C-33A2853842559054.0
HeLa243350962149.2
CL1–01743691453950.8
CL1–53646214180456.1
PANC-12243191947354.3
MIA-PaCa-22665222080150.2
A-4312994762366054.7
Jurkat195796211,26944.4
All 23 cell lines9981,4381212,02755.8
  • a Proteins predicted by the SignalP program to be secreted via the classical secretion pathway (SignalP probability ≥0.90).

  • b Proteins predicted to be secreted by the nonclassical secretion pathway using SignalP and SecretomeP (SignalP probability <0.90 and SecretomeP score ≥0.50).

  • c Proteins predicted by the TMHMM to form integral membrane proteins that were not predicted to be secreted via the classical or nonclassical secretion pathways.

  • d Proteins that could not be classified as classical secreted, nonclassical secreted, or integral membrane proteins.