Table I Proteins co-purified with ERα by IgG-Sepharose affinity chromatography and identified by two-dimensional PAGE followed by LC-MS/MS analysis

Following two-dimensional DIGE analysis of the TEV eluates shown in Fig. 3, the spots corresponding to protein species clearly detectable in ERα-containing samples only were cut, trypsin-digested, and subjected to LC-MS/MS analysis. The proteins identified, corresponding to spots A–D in the figure, are listed together with information relative to the MS data that led to their identification.

Protein ID (NCBI)Protein nameGene nameNo. of peptides matchedSequence coverageMowse score
%
Agi|119607896GelsolinGSN610230
Bgi|4501885β-ActinACTB619234
Cgi|10835063NucleophosminNPM11032477
Dgi|25013638NIa-Pro protein (tobacco etch virus)TEV protease210142