Table II Known β-actin-interacting proteins co-purified with ERα by IgG-Sepharose affinity chromatography and identified by nano-LC-MS/MS analysis

The list of β-actin-interacting proteins from the UniHI database was crossed with the list of proteins co-purified with TAP-ERα, but not with the TAP tag alone, and identified by nano-LC-MS/MS analysis of purified samples. Proteins are listed with information relative to the MS data that led to their identification. Two identified proteins not listed as validated direct interactors of actin in the UniHI database and ERα itself are reported in bold. n/a, not applicable.

Protein ID (Swiss-Prot)Protein nameGene nameNo. of peptides matchedSequence coverageMowse scoreConfirmed by WB
P03372Estrogen receptorESR12697n/a
P60709Actin, cytoplasmic 1ACTB20532509+
P68032Actin, α cardiac muscle 1ACTC114241901
P11142Heat shock cognate 71-kDa proteinHSPA8918294
Q0702060 S ribosomal protein L18RPL18524206
P17844Probable ATP-dependent RNA helicase DDX5DDX51422182+
P0538860 S acidic ribosomal protein P0RPLP0939180
P6242460 S ribosomal protein L7aRPL7A719113
P60660Myosin light polypeptide 6MYL6430104
P1812460 S ribosomal protein L7RPL762588
P6270140 S ribosomal protein S4, X isoformRPS4X82679
O00159Myosin 1cMYO1C9972+
O00571ATP-dependent RNA helicase DDX3XDDX3X4571
P4678140 S ribosomal protein S9RPS972962
P6224140 S ribosomal protein S8RPS842161
P61158Actin-related protein 3ACTR32758+
P61160aActin-related protein 2ACTR21132+