Table I S. aureus COL secreted proteins identified in present study

Numbers in the superscript indicate the different forms of the protein identified. Theor., theoretical; Sig P, signal peptide, seq., sequence.

Gene IDProtein nameTheor. massPredicted massaObserved massbMass errorModificationsTrap locationMS/MS seq. cov.c
DaDaDaDa%
Cytotoxins
    SACOL1173dα-Hemolysin precursor (HlY)35,973.333,260.133,260.60.5−Sig PTrap 684
    SACOL2003dβ-Hemolysin (Hlb)37,237.833,742.633,742.80.1−Sig PTrap 790
    SACOL20221eδ-Hemolysin (Hld1)2,976.6f2,976.6f2,976.6f0.0100
    SACOL20222eδ-Hemolysin (Hld2)2,976.6f3,020.6f3,020.6f0.0+N-terminal formylation, +16 Da100
    SACOL2419gγ-Hemolysin, component A (HlgA)34,955.731,921.9Trap 1053
    SACOL24221dγ-Hemolysin, component B (HlgB1)36,711.033,392.033,392.60.6−Sig P, truncated proteinTrap 469
    SACOL24222γ-Hemolysin, component B (HlgB2)36,711.033,462.133,463.61.5−Sig P, truncated proteinTrap 469
    SACOL24223γ-Hemolysin, component B (HlgB3)36,711.033,506.233,507.41.2−Sig P, truncated proteinTrap 469
    SACOL2421dγ-Hemolysin, component C (HlgC)35,625.832,565.232,566.21.0−Sig PTrap 985
    SACOL1880dLeukotoxin LukD (LukD)36,888.934,158.634,159.10.5−Sig PTrap 653
    SACOL1881dLeukotoxin LukE (LukE)34,819.131,750.431,751.51.1−Sig P, −C-terminal residue AsnTrap 868
    SACOL2004dLeukocidin subunit precursor, putative38,686.135,573.335,574.41.1−Sig PTrap 874
    SACOL20061d,hAerolysin/leukocidin family protein40,434.037,619.737,620.81.1−Sig PTrap 1072
    SACOL20062hAerolysin/leukocidin family protein40,434.037,418.437,419.30.9−Sig PTrap 1073
Superantigenic toxins
    SACOL0442Staphylococcal enterotoxin23,165.419,343.919,345.01.1−Sig PTrap 869
    SACOL0886dStaphylococcal enterotoxin (Sek)27,727.124,698.424,699.61.2−Sig P, −C-terminal residues YKETITrap 1282
    SACOL0887dStaphylococcal enterotoxin type I (Sei)28,184.624,846.624,848.01.4−Sig P, −C-terminal residues TETrap 2077
    SACOL0907dStaphylococcal enterotoxin B (Seb)31,435.828,368.028,368.50.5−Sig PTrap 8100
Proteases
    SACOL1869dSerine protease SplA (SplA)25,876.221,853.521,854.40.9−Sig PTrap 770
    SACOL1868dSerine protease SplB (SplB)26,096.422,371.122,372.00.9−Sig PTrap 666
    SACOL1867dSerine protease SplC (SplC)26,098.422,388.022,388.70.7−Sig PTrap 448
    SACOL1866dSerine protease SplD (SplD)25,669.122,010.822,012.21.4−Sig P, seq. errorTrap 577
    SACOL1865dSerine protease SplE (SplE)25,679.322,011.922,013.31.4−Sig PTrap 732
    SACOL1864dSerine protease SplF (SplF)25,655.121,941.721,942.71.0−Sig PTrap 593
    SACOL1057d,gV8 protease (SspA)36,312.633,376.1Trap 542
    SACOL1056dCysteine protease precursor SspB (SspB1)44,519.040,649.440,650.51.1−Sig PTrap 578
    SACOL1970d,gCysteine protease precursor SspB (SspB2)44,252.141,524.8Trap 1256
    SACOL2659d,gZinc metalloproteinase aureolysin (Aur)56,361.353,459.8Trap 848
Lipolytic enzymes
    SACOL2694dLipase1 (Lip1)76,675.373,077.373,078.00.8−Sig PTrap 896
    SACOL0317dLipase2 (Lip2)71,276.867,152.067,152.80.8−Sig PTrap 971
    SACOL0078d1-Phosphatidylinositol phosphodiesterase (Plc)37,086.734,127.134,128.00.9−Sig PTrap 690
Peptidoglycan hydrolases
    SACOL0263dPeptidoglycan hydrolase (LytM)35,067.431,735.531,736.51.0−Sig PTrap 466
    SACOL0507d,gN-Acetylmuramoyl-l-alanine amidase (Sle1)35,835.733,424.9Trap 1276
    SACOL07231dLysM domain protein28,186.811,848.911,849.00.1−Sig P, N-terminal fragmentTrap 2100
    SACOL07232LysM domain protein28,186.813,800.913,801.80.9−Sig P, C-terminal fragmentTrap 272
    SACOL10621dBifunctional autolysin (Atl1)137,334.9134,248.2134,249.51.3−Sig PTrap 999
    SACOL10622Bifunctional autolysin (Atl2)137,334.980,786.980,787.30.4−Sig P, proteolytic processingTrap 899
    SACOL10623Bifunctional autolysin (Atl3)137,334.953,479.553,479.70.2−Sig P, proteolytic processingTrap 999
    SACOL2088dSceD protein (SceD)24,096.021,497.021,497.70.7−Sig PTrap 376
    SACOL2584dImmunodominant antigen A (IsaA)24,203.221,377.921,377.5−0.4−Sig PTrap 391
    SACOL2666dN-Acetylmuramoyl-l-alanine amidase domain protein69,253.242,250.842,252.11.3−Sig P, protein degradationTrap 689
DaDaDaDa%
Miscellaneous enzymes
    SACOL0303dAcid phosphatase 5-nucleotidase33,351.930,184.330,185.00.7−Sig PTrap 1187
    SACOL0860Thermonuclease precursor (Nuc)25,119.918,782.318,782.0−0.3−Sig PTrap 797
    SACOL0962dGlycerophosphoryl diester phosphodiesterase (GlpQ)35,310.732,240.232,241.00.8−Sig PTrap 10100
    SACOL10711hChitinase-related protein11,344.88,720.88,721.00.2−Sig PTrap 391
    SACOL10712hChitinase-related protein11,344.88,906.98,907.10.2−Sig PTrap 389
    SACOL10713hChitinase-related protein11,344.89,902.09,901.6−0.4−Sig PTrap 392
Surface adhesins
    SACOL08581Secretory extracellular matrix and plasma-binding protein (Empbp1)38,484.912,890.512,891.81.3−Sig P, N-terminal fragmentTrap1573
    SACOL08582Secretory extracellular matrix and plasma-binding protein (Empbp2)38,484.922,709.822,708.9−1.0−Sig P, C-terminal fragmentTrap 1553
    SACOL0985dSurface protein, putative15,838.212,859.712,859.5−0.3−Sig PTrap 4100
    SACOL1164Fibrinogen binding-related protein12,596.69,592.19,592.50.4−Sig PTrap 369
    SACOL1168Fibrinogen-binding protein (Efb)18,764.615,850.115,851.00.9−Sig PTrap 1090
    SACOL2002Map protein (Map)76,945.273,877.7Trap 1731
    SACOL2019SdrH protein, putative (SdrH)46,630.038,083.838,085.11.2−Sig P, removal of C-terminal residues 377–419Trap 385
    SACOL2197dSurface protein, putative15,447.512,480.012,480.10.1−Sig PTrap 3100
    SACOL2418dIgG-binding protein (Sbi)50,070.247,049.747,049.3−0.4−Sig PTrap 995
    SACOL2660Immunodominant antigen B (IsaB)19,370.215,785.015,785.30.3−Sig PTrap 3100
Unknown functions
    SACOL0270dStaphyloxanthin biosynthesis protein, putative33,032.230,379.130,377.9−1.2−Sig P, −42-Da modificationTrap 1259
    SACOL0271Virulence factor EsxA (EsxA)11,306.210,905.210,905.20.2−MetTrap 3100
    SACOL0480Hypothetical protein11,301.88,380.38,380.40.1−Sig PTrap 9100
    SACOL0669dConserved hypothetical protein18,594.215,905.015,905.40.4−Sig PTrap 6100
    SACOL0755dConserved hypothetical protein16,922.113,449.913,449.1−0.8−Sig PTrap 779
    SACOL0859Hypothetical protein17,717.014,812.514,813.40.9−Sig PTrap 767
    SACOL0908dHypothetical protein20,345.816,271.116,271.10.0−Sig PTrap 599
    SACOL1166dHypothetical protein15,202.512,304.012,304.00.0−Sig PTrap 555
    SACOL2179Conserved hypothetical protein32,763.629,134.029,135.51.5−Sig PTrap 842
    SACOL22911dStaphylococcal secretory antigen SsaA229,327.112,211.312,210.3−1.0−Sig P, N-terminal fragmentTrap 398
    SACOL22912Staphylococcal secretory antigen SsaA229,327.114,294.514,294.90.4−Sig P, C-terminal fragmentTrap 397
    SACOL2295dStaphyloxanthin biosynthesis protein17,424.814,755.714,756.71.0−Sig P, +16-Da modificationTrap 371
    SACOL2557Conserved domain protein16,870.312,788.512,789.51.0−Sig PTrap 661
  • a Predicted masses were calculated by subtracting the mass of the signal peptide from the theoretical mass. For proteins with additional modifications, recalculated predicted mass is presented in the table.

  • b Average mass of the protein from replicate analyses.

  • c Net sequence coverage of the mature protein.

  • d Protein identified in other S. aureus strains (29).

  • e Protein identified in the extracellular peptide extract by peptide MS/MS analysis; the reported protein mass was calculated from precursor ion mass.

  • f Monoisotopic mass.

  • g Protein could not be identified by whole protein MS analysis.

  • h Protein with signal peptide cleavages at more than one site.