No. | m/z | Charge | Mascot score | MHC I allele | Peptide sequence | Gene ID | Gene symbol | Gene Name | WT/dKO ratio |
---|---|---|---|---|---|---|---|---|---|
1 | 520.22 | 2 | 66 | H2Db | GGVVNMYHM | 16913 | Psmb8 | Proteasome subunit, beta type 8 (LMP7) | ≥1181,3 |
2 | 460.25 | 2 | 40 | H2Kb | ATRSFPQL | 11750 | Anxa7 | Annexin A7 | ≥380,8 |
3 | 624.83 | 2 | 44 | H2Db | HAIRNSFQYL | 170749 | Mtmr4 | Myotubularin related protein 4 | ≥207,3 |
4 | 472.28 | 2 | 55 | H2Kb | TIIIFHSL | 14050 | Eya3 | Eyes absent 3 homolog (Dm) | 160,5 |
5 | 497.27 | 2 | 43 | H2Kb | VVYIYHSL | 67014 | Mina | Myc induced nuclear antigen | ≥159,3 |
6 | 467.28 | 2 | 47 | H2Kb | SALRFLNL | 71728 | Stk11ip | Ser/Thr kinase 11 interacting protein | 120,8 |
7 | 479.28 | 2 | 39 | H2Kb | KNVLFSHL | 19084 | Prkar1a | Protein kinase, cAMP dependent regulatory, type I, alpha | ≥109,8 |
8 | 524.27 | 2 | 65 | H2Db | RQILNADAM | 66185 | 1110037F02Rik | RIKEN cDNA 1110037F02 gene | ≥96,8 |
9 | 496.78 | 2 | 42 | H2Db | SGLLNMTKI | 216445 | Arhgap9 | Rho GTPase activating protein 9 | ≥93,9 |
10 | 525.26 | 2 | 29 | H2Kb | HVYYFAHL | 74558 | Gvin1 | GTPase, very large interferon inducible 1 | ≥93,4 |
11 | 510.80 | 2 | 56 | H2Kb | VITNFSARI | 68505 | 1110014N23Rik | RIKEN cDNA 1110014N23 gene | ≥83,3 |
12 | 507.26 | 2 | 49 | H2Kb | STLIYRNM | 75660 | Lin37 | Lin-37 homolog (C. elegans) | ≥82,1 |
13 | 597.28 | 2 | 27 | H2Db | SAIHNFYDNI | 68652 | Map3k7ip2 | MAPKKK 7 interacting protein 2 | ≥80,5 |
14 | 523.75 | 2 | 34 | H2Db | SQVYNDAHI | 66923 | Pbrm1 | Polybromo 1 | ≥76,9 |
15 | 537.27 | 2 | 40 | H2Kb | LVYQFKEM | 56501 | Elf4 | E74-like factor 4 (ets domain transcription factor) | ≥76,2 |
16 | 461.76 | 2 | 62 | H2Db | GAVQNIAHL | 74125 | Armc8 | Armadillo repeat containing 8 | 68,5 |
17 | 496.28 | 2 | 32 | H2Kb | ISLEFRNL | 14137 | Fdft1 | Farnesyl diphosphate farnesyl transferase 1 | 68,1 |
18 | 506.30 | 2 | 43 | H2Kb | KSYLFQLL | 12566 | Cdk2 | Cyclin-dependent kinase 2 | ≥66,4 |
19 | 447.74 | 2 | 53 | H2Db | GGIQNVGHI | 23918 | Impdh2 | Inosine 5′-phosphate dehydrogenase 2 | 54,8 |
20 | 493.24 | 2 | 41 | H2Kb | SNYRFEGL | 319955 | Ercc6 | Excision repair cross-complementing rodent repair deficiency, complementation group 6 | ≥46,4 |
21 | 479.75 | 2 | 37 | Qa2 | GLMTTVHAI | 640374 | LOC640374 | Similar to GAPDH | 42,0 |
22 | 645.34 | 2 | 33 | H2Db | AQYGNILKHVM | 69482 | Nup35 | Nucleoporin 35 | ≥40,3 |
23 | 486.78 | 2 | 48 | H2Db | KAPLNIAVM | 667214 | RP23–269N23.3 | Similar to immunity-related GTPase family, cinema 1 | 39,8 |
24 | 510.81 | 2 | 54 | H2Db | YGLLNVTKI | 75415 | Arhgap12 | Rho GTPase activating protein 12 | ≥37,6 |
25 | 501.27 | 2 | 42 | H2Db | SALANYIHL | 58245 | Gpr180 | G protein-coupled receptor 180 | ≥37,4 |
MIPs that were not detected on dKO cells were attributed the intensity value 15,000, which represents the threshold for limit of detection. Ratios with the symbol ≥ indicate peptides for which the exact fold change could not be measured because they were detected only in WT DCs. See supplemental Table S1 for a complete list of MIPs detected on DCs.