Table II Spectra, peptides, and proteins identified and quantified of eight independent iTRAQ experiments
ExperimentiTRAQMass spectrometerQuantityTotal spectraIdentified peptides (confidence = 95%)Identified peptides (confidence = 95%, contribution = 2)Identified proteins (after grouping)Quantified proteins (unused >4)
Unused >2Unused >4
μg
iTRAQ-14-plex4800100146,06351,39917,287217512101180
iTRAQ-24-plex4800100105,01352,4626638201711271102
iTRAQ-74-plex5800100229,54588,1068215225112531224
iTRAQ-88-plex5800100338,26267,6066573193710411012
iTRAQ-68-plex4800100131,14342,00512,9971406742740
iTRAQ-34-plex48007086,38023,76882991214604603
iTRAQ-44-plex48007097,11046,23468441075557557
iTRAQ-54-plex48007057,18626,24739451242431431