Table I Samples used for the differential gel electrophoresis (DIGE) analysis. Mesenchymal stem cells from 12 donors were differentiated toward chondrocyte-like cells. The top of the table indicates the amount of protein used in DIGE experiments labeled with Cy2, Cy3, or Cy5 dye. The first column indicates the gel number; the following columns indicate the days in differentiation medium charged in the gel. Three different experiments were performed and are indicated as *, ′, and ″. Each experiment utilized a pool of three chondrogenic differentiations from three different donors. Standard is the mix of same amount of protein of each condition
Cy2 (50 μg)Cy3 (50 μg)Cy5 (50 μg)
GEL1Standard0*4*
GEL2Standard7*14*
GEL3Standard28*46*
GEL4Standard4′0′
GEL5Standard14′7′
GEL6Standard46′28′
GEL7Standard0″4″
GEL8Standard7″14″
GEL9Standard28″46′