Table II Proteomic analysis at different times of chondrogenesis from mesenchymal stem cells (MSCs) from human umbilical cord stroma using a two-dimensional DIGE technique coupled to MALDI-TOF/TOF. Only those proteins modulated during chondrogenesis identified by at least two specific human peptides with confidence >99% are included in the list
NAMEMSMS matchLocationScore# ID peptidesSpotp anova
Annexin A2ANXA2_HUMANExtracellular matrix973157752.58E-01
Peroxiredoxin-5PRDX5_HUMANMitochondrion47575656.73E-01
GRP78GRP78Endoplasmic reticulum772176560.001
Protein disulfide-isomerase A3PDIA3_HUMANEndoplasmic reticulum872136750.001
Tropomyosin beta chainTPM2_HUMANCytoplasm19047590.002
TransgelinTAGL_HUMANCytoplasm673156370.002
Heat shock protein, mitochondrialCH60_HUMANMitochondrion75310910.002
VimentinVIME_HUMANIntermediate filament25297510.002
Protein DJ-1PARK7_HUMANNucleus27098330.003
Protein SETSET_HUMANEndoplasmic reticulum6626930.003
CalreticulinCALR_HUMANEndoplasmic reticulum55185780.003
Reticulocalbin-3RCN3_HUMANEndoplasmic reticulum42376550.004
Annexin A5ANXA5_HUMANExtracellular matrix644127880.004
Annexin A1ANXA1_HUMANExtracellular matrix1329157760.004
TransgelinTAGL_HUMAN3711510890.005
Annexin A1ANXA1_HUMANExtracellular matrix1029157720.005
Heat shock protein beta-1HSPB1_HUMANCytoplasm58588490.005
EndoplasminENPL_HUMANEndoplasmic reticulum542125260.005
Annexin A6ANXA6_HUMANExtracellular matrix70833910.005
Procollagen-lysinePLOD2_HUMANEndoplasmic reticulum membrane18432210.008
Reticulocalbin-1RCN1_HUMANEndoplasmic reticulum18746950.008
Stress-induced-phosphoprotein 1STIP1_HUMANCytoplasm & nucleus12256710.008
Peptidyl-prolyl cis-trans isomerase APPIA_HUMANCytoplasm550812500.009
Annexin A1ANXA1_HUMANExtracellular matrix874156320.009
Peptidyl-prolyl cis-trans isomerase APPIA_HUMANCytoplasm508812850.011
Annexin A5ANXA5_HUMANExtracellular matrix6911210810.011
ZyxinZYX_HUMANCytoplasm & nucleus20661860.011
Annexin A2ANXA2_HUMANExtracellular matrix396137830.013
Superoxide dismutaseSODM_HUMANMitochondrion39158640.014
Phosphoglycerate mutase 1PGAM1_HUMANCytoplasm38188430.015
Ubiquitin carboxyl-terminal hydrolase isozyme L1UCHL1_HUMANCytoplasm22388960.017
Ferritin light chainFRIL_HUMANCytoplasm130413050.017
Nucleoside diphosphate kinase ANDKA_HUMANCytoplasm & Nucleus269612780.018
Annexin A2ANXA2_HUMANExtracellular matrix492137810.019
Superoxide dismutaseSODM_HUMANMitochondrion130510820.020
Protein disulfide-isomerase A3PDIA3_HUMANEndoplasmic reticulum457136720.031
Tyrosyl-tRNA synthetase, cytoplasmicSYYC_HUMANCytoplasm6424690.033
Glyceraldehyde-3-phosphate dehydrogenaseG3P_HUMANMembrane6235090.033
Transgelin-2TAGL2_HUMANMembrane8091410750.033
Heat shock protein beta-1HSPB1_HUMANCytoplasm & Nucleus40589780.034
Nicotinamide N-methyltransferaseNNMT_HUMANCytoplasm486108730.034
CalumeninCALU_HUMANEndoplasmic reticulum44876800.035
Peroxiredoxin-6PRDX6_HUMAN38178480.039
Annexin A5ANXA5_HUMANExtracellular matrix277126340.039
Triosephosphate isomeraseTPIS_HUMANCytoplasm1061148580.039
Alpha-enolaseENOA_HUMAN63196500.040
Annexin A2ANXA2_HUMANExtracellular matrix643137740.043
Glutathione S-transferase PGSTP1_HUMANCytoplasm43969510.045
CaldesmonCALD1_HUMANPlasma membrane28082830.046
VimentinVIME_HUMANIntermediate filament19396730.046
Protein disulfide-isomerase A1PDIA1_HUMANEndoplasmic reticulum1045136160.046
Protein disulfide-isomerase A3PDIA3_HUMANEndoplasmic reticulum543136150.048
Heterogeneous nuclear ribonucleoprotein d-likeHNRDL_HUMANCytoplasm & Nucleus20447700.048
CaldesmonCALD1_HUMANPlasma membrane26782750.050