Table I Phosphopeptides that significantly changed in abundance in response to dDAVP

In the peptide sequences, # indicates a phosphorylation site, and @ indicates methionine oxidation.

Protein nameRefSeqGene symbolPeptide sequencePhosphositeAverage log2(dDAVP/control)
0.5 min2 min5 min15 min
Average log2(dDAVP/control) ≥ 0.5
    1-Phosphatidylinositol 4,5-bisphosphate phosphodiesterase β-3NP_203501Plcb3NNS#ISEAKSer-11070.410.68b0.65b0.67
    2-Oxoisovalerate dehydrogenase subunit α, mitochondrialNP_036914BckdhaIGHHS#TSDDSSAYRSer-338a−0.16−0.26−0.071.00b
    6-Phosphofructo-2-kinase/fructose-2,6-biphosphatase 3NP_476476Pfkfb3NSVTPLAS#PEPTKSer-496−0.260.76b−0.080.40
    α1-SyntrophinNP_001094371Snta1NSAGGTSVGWDS#PPASPLQRSer-183a−0.090.56−0.331.12b
    Ankyrin repeat and SOCS box protein 4NP_001019489Asb4S#LPLSLKSer-4081.40b1.96b1.201.24b
    Aquaporin-2NP_037041Aqp2RQS#VELHS#PQSLPRSer-256, Ser-2610.720.66b0.83−0.15
    Aquaporin-2NP_037041Aqp2RQS#VELHSPQSLPRSer-2560.890.83b1.091.13
    Bcl-2-related ovarian killer proteinNP_059008BokRSS#VFAAEIMDAFDRSer-81.991.212.92b2.28b
    Calcium/calmodulin-dependent protein kinase kinase 2NP_112628Camkk2S#FGNPFEGSRSer-4940.260.22b0.46b0.76b
    cAMP-dependent protein kinase type I-α regulatory subunitNP_037313Prkar1aTDS#REDEISPPPPNPVVKSer-77a0.65b−0.160.160.32
    Cytokine receptor-like factor 2 precursorNP_604460Crlf2GS#FPGLFEKSer-278−0.10.410.260.59b
    Focal adhesion kinase 1NP_037213Ptk2LQPQEIS#PPPTANLDRSer-9130.17−0.010.51b0.20
    Hypothetical proteinXP_001079852Fam83eETPTTTGPALS#DILRSer-3631.19b0.161.700.47
    Keratin, type I cytoskeletal 19NP_955792Krt19GGS#FSGALTVTDGLLGGNEKSer-670.08−0.110.19b0.60b
    Kinesin-like protein KIF13BNP_998791Kif13bSS#GLQPQGAPEARSer-1719a1.09b0.99b0.83b1.07b
    Leucine-rich repeat flightless-interacting protein 1NP_001014291Lrrfip1RGS#GDTSISM@DTEASIRSer-880.641.22b0.670.80b
    Leucine-rich repeat flightless-interacting protein 2NP_001019932Lrrfip2NSASATTPLSGNSS#RSer-129a0.27b0.70b0.53b0.67b
    Lipopolysaccharide-responsive and beige-like anchor proteinNP_001102025LrbaQETAPDT#GDQQRThr-12310.42b0.330.52b0.51b
    Mitogen-activated protein kinase kinase kinase 7NP_001101390Map3k7S#IQDLTVTGTEPGQVSSRSer-4390.62b0.55b0.640.60b
    Nuclear receptor coactivator 3XP_001072953Ncoa3ALS#LDSPVSVGSVPPVKSer-8480.55bc
    OCIA domain-containing protein 1NP_001013896Ociad1S#VTYEELRSer-1980.540.580.700.67b
    Phosphatidylinositol glycan anchor biosynthesis, class BNP_001101636PigbS#TQYVIAQEKSer-291.001.23b0.840.83
    Rap1 GTPase-activating proteinXP_001070178Rap1gapSQS#M@DAM@GLSNKSer-4721.190.78b0.65b1.08
    Signal transducer and activator of transcription 5ANP_058760Stat5aLS#PPAGLFTSARSer-779−0.560.380.56b
    Similar to Map4k6-pending proteinXP_002724558LOC303259SNS#AWQIYLQRSer-7140.82b0.75b0.83b0.70b
    Splicing factor, proline- and glutamine-richNP_001020442SfpqGM@GPGT#PAGYGRThr-679−0.370.340.070.87b
    TBC1 domain family, member 1XP_001071842Tbc1d1S#LTESLESILSRSer-4970.56b0.91b0.85b1.08b
    Tyrosine-protein phosphatase nonreceptor type 12NP_476456Ptpn12DADVSEES#PPPLPERSer-667−0.201.35−0.130.72b
    Urea transporter 2 isoform 1NP_062220Slc14a2S#VFHIEWSSIRSer-4861.65b2.00b1.95b1.98b
    Urea transporter 2 isoform 2NP_808877Slc14a2RRES#ELPRSer-841.611.81b1.74b1.85b
    Vesicle-trafficking protein SEC22bNP_001020857Sec22bNLGS#INTELQDVQRSer-1373.020.682.901.75b
Average log2(dDAVP/control) ≤ −0.5
    AHNAK nucleoprotein isoform 1XP_001078032AhnakGDVAASS#PSMKSer-4506−0.23−0.55-0.58b-0.78b
    α-Endosulfine isoform 1NP_001029146EnsaYFDS#GDYNMAKSer-67−0.18−0.51b−0.32−0.35
    AlsinNP_001013431Als2LSLPGLLSQVS#PRSer-486−0.29−0.55b
    Aquaporin-2NP_037041Aqp2QSVELHS#PQSLPRSer-261−2.79b−3.32b−3.03b−2.99b
    Arf-GAP domain and FG repeats-containing protein 1NP_001129068Agfg1GTPTQS#PVVGRSer-181−0.24b−0.53−0.60−0.56b
    Catenin α-1NP_001007146Ctnna1TPEELDDS#DFETEDFDVRSer-6430.11−0.19−0.57b−0.21
    CLIP-associating protein 2NP_446174Clasp2MVSQSQPGS#RSer-465−0.79b0.390.12−0.14
    Epidermal growth factor receptor kinase substrate 8-like protein 1NP_001101937Eps8l1GASPAAET#PPLQRThr-187−0.22−0.17−0.60−0.72b
    Erythrocyte protein band 4.1-like 4aNP_001100867Epb4.1l4aVAQTQPAGSNS#INRSer-182−0.08−0.50b0.07−0.20
    Hypothetical protein LOC500552NP_001128101RGD1561149CSLLSAS#PASVRSer-326−0.67b−0.240.48−0.46
    Plastin-2NP_001012044Lcp1GS#VSDEEMM@ELRSer-5−0.64b0.24−0.321.20
    Septin-9 isoform 2NP_789826Sept9QVESTAST#PGPSRThr-107−0.74−0.90−0.39b−0.88b
    Serine/threonine-protein kinase PAK 2NP_445758Pak2IIS#IFSSTEKSer-55a−0.29−0.58b−0.52−0.10
    Tumor protein p53 binding protein, 2 isoform 1XP_001063503Tp53bp2ENLPVS#PDGNPPQQAVSAPSRSer-325−1.06b−0.47−0.11−0.35
  • a Ambiguous phosphorylation site.

  • b p < 0.05.

  • c —, no quantifiable iTRAQ ratio.