Table I Crosslinks within the ATPase domain of ISWI
ConstructCrosslinked peptides
Mass (Da)Error (ppm)Bpa peptideTarget peptide
Sequencea,bSiteSequencebSite
XL1ISWI26–6482734.3062+0.7STLBNWVNEFK190AVCLIGDQDTR209
XL2Q190B2862.4012+0.3STLBNWVNEFKK190AVCLIGDQDTR209
XL3ISWI26–6481826.9779+0.4IBVGLSK361GEAKTAEQKc631–632c
XL4F361B2014.0875+0.7IBVGLSK361LITESTVEEK555
XL51309.6932+0.1BVIQGGR578HFK172
XL6ISWI26–6481522.8621+0.1BVIQGGR578LRLDK573
XL7aM578B1940.9845+3.7BVIQGGR578AALDSLGESSL644
XL7b1940.9845+3.7BVIQGGR578AALDSLGESSL645
XL83163.6562+2.7BVIQGGR578LVDNRSNQLNKDEMLNIIR589
XL91309.6932+7.1BVIQGGR578HFK172
XL101494.7944+5.1BVIQGGR578LVDNR585
XL111522.8621+5.6BVIQGGR578LRLDK573
XL12a2097.0856+6.3BVIQGGR578AALDSLGESSLR644
XL12bISWI FLd2097.0856+6.3BVIQGGR578AALDSLGESSLR645
XL13M578B2246.1737+8.7LDKBVIQGGR578FDASPAYIK120
XL142453.2916+9.0LDKBVIQGGR578AALDSLGESSLR644
XL152640.4793+9.0BVIQGGR578TLQTISLLGYLKHFK172
XL162722.4768+5.0LRLDKBVIQGGR578AALDSLGESSLR644
XL173163.6562+7.7BVIQGGR578LVDNRSNQLNKDEMLNIIRc589–591c
  • a B symbolizes Bpa.

  • b Crosslinked amino acids are underlined.

  • c Precise attachment sites not distinguishable from data.

  • d Real time calibration by lock masses not available leading to an increased error.