Table I Bold and underlined residue depicts glycan attachment site, underlined residues depict experimentally indistinguishable attachment sites. * p < 0.05.

List of urinary glycoproteins identified from their glycopeptide sequences, glycan structures and exact attachment sites.

Uniprot/KB accessionGlycoproteinPeptide sequenceAttachment siteGlycanMascot scoreMascot threshold*ECD
O-linked Glycopeptides
    P02765Alpha-2-HS-glycoproteinR. TVVQPSVGAAAGPVVPPCPGR.I346HexHexNAc25>18Yes
    P02656Apolipoprotein C-IIID.PEVRPTSAVAA.-94HexHexNAc19>16Yes
    Q13790Apolipoprotein FK.DANISQPETTKEGLR.A256bHexHexNAc57>21Yes
    P98160Basement membrane-specific heparan sulfate proteoglycan core proteinR.AYDGLSLPEDIETVTASQMR.W42bHexHexNAc93>20Yes
    P26842CD27 antigenD.PLPNPSLTAR.S127bHexHexNAc16>14Yes
    P16070CD44 antigenS.QEGGANTTSGPIR.T637–638bHexHexNAc43>33Yes
    P00742Coagulation factor XR.SVAQATSSSGEAPDSITWKPYDAADLD.P183–203HexHexNAc41>38No
    P00742Coagulation factor XK.SHAPEVITSSPL.K476–485bHexHexNAc37>33No
    P39059Collagen alpha-1(XV) chainE.ILEAVTYTQASPK.E265bHexHexNAc68>32Yes
    P10643Complement component C7N.PLTQAVPK.C696bHexHexNAc39>29No
    Q68CJ9Cyclic AMP-responsive element-binding protein 3-like protein 3R.VAADAVPGSEAPGPRPEADTTR.E379bHexHexNAc47>21Yes
    Q13508Ecto-ADP-ribosyltransferase 3K.SQGNINNPTPGPVPVPGPK.S346bHexHexNAc59>20Yes
    P98095Fibulin-2R.AEAGARPEENLILDAQATSR.S347–348bHexHexNAc54>24No
    Q8NFU4Follicular dendritic cell secreted peptideR.RNFPIPIPESAPTTPLPSE.K75–83bHexHexNAc64>31No
    P78423FractalkineK.AQDGGPVGTELFR.V183bHexHexNAc79>22Yes
    P78423FractalkineR.VWGQGQSPRPENSLER.E253HexHexNAc35>21Yes
    P78423FractalkineR.LGVLITPVPDAQAATR.R329–338HexHexNAc43>13No
    P04921Glycophorin-CD.PGMSGWPDGR.M42aHexHexNAc59>30No
    Q8TDQ0Hepatitis A virus cellular receptor 2R.DFTAAFPR.M145bHexHexNAc32>21No
    P04233HLA class II histocompatibility antigen gamma chainS.LEQKPTDAPPK.V203bHexHexNAc37>32No
    P04233HLA class II histocompatibility antigen gamma chainD.PSSGLGVTKQDLGPVP.M.-281–287bHexHexNAc42>40No
    P01344Insulin-like growth factor IIR.DVSTPPTVLPDNFPR.Y96b, 99aHex2HexNAc267>21Yes
    P01344Insulin-like growth factor IIR.DVSTPPTVLPDNFPR.Y99aHexHexNAc63>21Yes
    P01344Insulin-like growth factor IIR.DVSTPPTVLPDNFPR.Y96b–99aHexHexNAc239>21Yes
    P01344Insulin-like growth factor IIP.LIALPTQD.P163HexHexNAc(38)>42No
    P19823Inter-alpha-trypsin inhibitor heavy chain H2K.VVPDSTPSWANPSPTPVISML.A665–679bHex3HexNAc3(31)>33No
    Q14624Inter-alpha-trypsin inhibitor heavy chain H4K.IEETTMTTQTPAPIQAPSAILPLPGQSVER.L720b, 722–723bHex2HexNAc249>18Yes
    Q14624Inter-alpha-trypsin inhibitor heavy chain H4K.IEETTMTTQTPAPIQAPSAILPLPGQSVER.L719–725bHex3HexNAc330>17Yes
    O95998Interleukin-18-binding proteinD.PCPSQPPVFPAAK.Q53Hex2HexNAc217>17No
    O95998Interleukin-18-binding proteinD.PCPSQPPVFPAAK.Q53HexHexNAc60>33Yes
    P09603Macrophage colony-stimulating factor 1K.GQQPADVTGTALPR.V363b, 365bHex2HexNAc257>20No
    P09603Macrophage colony-stimulating factor 1R.ISSLRPQGLSNPSTLSAQPQLSR.S406–426bHexHexNAc55>22No
    Q13361Microfibrillar-associated protein 5D.PATDETVLA.V54bHexNAc(37)>40Yes
    Q13361Microfibrillar-associated protein 5D.PATDETVLA.V54bHexHexNAc42>42Yes
    Q13361Microfibrillar-associated protein 5D.PATDETVLA.V54bHexHexNAc + Sulf42>38No
    Q6UXB8Peptidase inhibitor 16E.LQATLDHTGHTSSK.S386–395bHexHexNAc34>33No
    Q96FE7Phosphoinositide-3-kinase-interacting protein 1R.EDQTSPAPGLR.C39bHexHexNAc51>20Yes
    P05155Plasma protease C1 inhibitorK.VATTVISK.M47–48aHexHexNAc21>8Yes
    P05155Plasma protease C1 inhibitorK.VATTVISK.M47b, 48aHex2HexNAc216>8Yes
    P05154Plasma serine protease inhibitorR.VEDLHVGATVAPSSR.R39bHexHexNAc66>20Yes
    P01133Pro-epidermal growth factorK.NQVTPLDILSK.T801–807bHexHexNAc47>20No
    P01133Pro-epidermal growth factorR.LSEPGLICPDSTPPPHLR.E954–955bHexHexNAc57>20Yes
    P01133Pro-epidermal growth factorR.LSEPGLICPDSTPPPHLR.E954–955bHex2HexNAc272>20Yes
    Q99075Proheparin-binding EGF-like growth factorD.PPTVSTDQLLPLGGGR.D44bHexHexNAc86>31Yes
    Q99075Proheparin-binding EGF-like growth factorD.PPTVSTDQLLPLGGGR.D44b, 47bHex2HexNAc261>31Yes
    Q9UHG2ProSAASR.GLSAASPPLAETGAPR.R53HexHexNAc60>21Yes
    Q9UHG2ProSAASR.AADHDVGSELPPEGVLGALLR.V228bHexHexNAc84>20Yes
    Q9UHG2ProSAASK.RLETPAPQVPAR.R247HexHexNAc42>17Yes
    P80370Protein delta homolog 1R.ALSPQQVTR.L256bHexHexNAc30>27Yes
    Q9ULI3Protein HEG homolog 1R.EPPPTPPRER.R67bHexHexNAc25>23No
    Q9GZM5Protein YIPF3K.AVAVTLQSH.-346bHexNAc43>19Yes
    Q9GZM5Protein YIPF3K.AVAVTLQSH.-346bHexHexNAc32>19Yes
    Q9GZM5Protein YIPF3K.AVAVTLQSH.-346bHexHexNAc243>21Yes
    Q9GZM5Protein YIPF3K.AVAVTLQSH.-346bHex2HexNAc233>20Yes
    Q9GZM5Protein YIPF3K.AVAVTLQSH.-346bdHexHex2HexNAc2(8)>18Yes
    Q16849Receptor-type tyrosine-protein phosphatase-like NK.AARPPVTPVLLE.K441bHexHexNAc21>20Yes
    Q4LDE5Sushi, von Willebrand factor type A, EGF and pentraxin domain-containing protein 1Y.DDFLDTVQETATSIGNAK.S887–894bHexHexNAc119>33No
    P34741Syndecan-2K.IPAQTKSPEETDK.E101bHexHexNAc30d>25dYes
    Q6UWD8Transmembrane protein C16orf54M.PLTPEPPSGR.V4bHexHexNAc30>22Yes
    P25445Tumor necrosis factor receptor superfamily member 6A.QVTDINSK.G28bHexHexNAc30>29Yes
    Q9UFP1Protein FAM198AD.PGPMEPQGVTGAPATHIR.Q53–58bHexHexNAc77>41No
    P04070Vitamin K-dependent protein CG.TPAPLDSVFSSSER.A19bHexHexNAc67>34Yes
    P04070Vitamin K-dependent protein CG.TPAPLDSVFSSSER.A + Phosphorylation19bHexHexNAc49>39Yes
N-linked Glycopeptides
P02763Alpha-1-acid glycoprotein 1N.LVPVPITNATLDQITGK.W33Hex6HexNAc533>17No
    P19652Alpha-1-acid glycoprotein 2N.LVPVPITNATLDR.I33Hex6HexNAc522>17No
    P01009Alpha-1-antitrypsinR.QLAHQSNSTNIFFSPVSIATAFAMLSLGTK.A70Hex5HexNAc419>14No
    P01009Alpha-1-antitrypsinK.YLGNATAIFFLPDEGK.L271Hex5HexNAc431>15No
    P02765Alpha-2-HS-glycoproteinK.VCQDCPLLAPLNDTR.V156Hex5HexNAc426>15No
    P02765Alpha-2-HS-glycoproteinF.NAQNNGSNFQLEEISR.A176Hex5HexNAc435>27No
    P05090Apolipoprotein DR.CIQANYSLMENGK.I65aHex6HexNAc536>8No
    P05090Apolipoprotein DR.ADGTVNQIEGEATPVNLTEPAK.L98dHex1Hex7HexNAc618>10No
    Q96IY4Carboxypeptidase B2C.SVLLADVEDLIQQQISNDTVSPR.A108aHex5HexNAc420>19No
    P01876Ig alpha-1 chain C regionR.PALEDLLLGSEANLTCTLTGLR.D144Hex5HexNAc43123No
    O95998Interleukin-18-binding proteinR.FPNFSILYWLGNGSFIEHLPGR.L103bHex5HexNAc410>4No
    O95998Interleukin-18-binding proteinK.ALVLEQLTPALHSTNFSCVLVDPEQVVQR.H147bdHex1Hex5HexNAc419>11No
    Q96FE7Phosphoinositide-3-kinase-interacting protein 1R.CLNWLDAQSGLASAPVSGAGNHSYCR.N66bdHex1Hex5HexNAc436>10No
    P05155Plasma protease C1 inhibitorS.NPNATSSSSQDPESLQDR.G25aHex5HexNAc469>22No
    P15151Poliovirus receptorR.VEDEGNYTCLFVTFPQGSR.S120aHex5HexNAc428>7No
    P41222Prostaglandin-H2 d-isomeraseK.SVVAPATDGGLNLTSTFLR.K78dHex1Hex5HexNAc413>6No
    P02760Protein AMBPK.WNITMESYVVHTNYDEYAIFLTK.K115Hex5HexNAc415>13No
    P02760Protein AMBPR.YFYNGTSMACETF.Q250aHex5HexNAc421>13No
    P00734ProthrombinR.GHVNITR.S121bHex5HexNAc419>4Yes
    P00734ProthrombinR.YPHKPEINSTTHPGADLQENFCR.N143bHex5HexNAc430d>18dYes
    P07911UromodulinR.CNTAAPMWLNGTHPSSDEGIVSR.K232aHex7HexNAc617>15No
    P07911UromodulinK.QDFNITDISLLEHR.L322adHex1Hex7HexNAc613>10No
    P07911UromodulinR.NETHATYSNTLY.L396aHex6HexNAc519>19No
    Q6EMK4VasorinR.LHEITNETFR.G117aHex5HexNAc4(11)d>15dYes
    P25311Zinc-alpha-2-glycoproteinR.FGCEIENNR.S128aHex5HexNAc414>11No
  • a Site occupancy reported in the UniProtKB/Swiss-Prot database, glycan unknown.

  • b Site occupancy and glycan not reported in the UniProtKB/Swiss-Prot database.

  • c For N-glycan microheterogeneity, see supplementary Table III.

  • d Mascot score and threshold values obtained for ECD data.