Table II Selected Plk1-regulated phosphorylation sites
ProteinShort nameaSiteSequencebPlk1asPlk1wt
Average ratiocNo. ratiosdAverage ratiocNo. ratiosd
Centrosomal protein of 55 kDaCEP55Ser436eTAALNESLVECPK7.7240.944
NucleophosminNPM1Ser4fMEDSMDMDMS2.4140.984
FAS-associated death domain proteinFADDSer194gNRSGAMSPMSWNS12.8820.902
Rac GTPase-activating protein 1MgcRacGAPSer154ESGSILSDISFDK10.3930.942
Rac GTPase-activating protein 1MgcRacGAPSer157hSILSDISFDKTDE11.8330.992
Rac GTPase-activating protein 1MgcRacGAPSer170hSLDWDSSLVKTFK3.8930.743
Protein NEDD1NEDD1Ser404iKNQDFSSFDDTGK1.7930.994
Anaphase-promoting complex subunit 1APC1Ser377jISSHNQSPKRHSI5.7540.994
Cell division cycle protein 16 homologCdc16Ser112jKYLKDESGFKDPS20.1921.034
Cell division cycle protein 27 homologCdc27Ser426jQPNINDSLEITKL18.0640.984
Cell division cycle protein 27 homologCdc27Ser435jITKLDSSIISEGK9.3740.994
Cell division cycle protein 27 homologCdc27Ser387LFTSDSSTTKENS2.1241.013
DNA topoisomerase 2αTOP2AThr1280KIKNENTEGSPQE5.1321.114
Mitotic checkpoint serine/threonine-protein kinase BUB1βBubR1Thr710PEKLELTNETSEN13.6220.831
Nuclear mitotic apparatus protein 1NuMA-1Ser1789QAPLESSLDSLGD3.9720.923
E3 SUMO-protein ligase RanBP2RanBP2Ser2270TTGFNFSFKSALS6.9330.944
StathminSTMN1Ser46PKKKDLSLEEIQK1.7141.004
M-phase inducer phosphatase 3Cdc25CSer191kDQAEEISDELMEF8.8220.882
E3 ubiquitin-protein ligase HUWE1HUWE1Ser1368GVVRDLSMSEEDQ4.4141.014
Suppressor of G2 allele of SKP1 homologSUGT1Ser331KRAMNKSFMESGG4.3821.003
F-box only protein 30FBXO30Ser213IGMLNTSVPNDMD6.1620.973
Ubiquitin carboxyl-terminal hydrolase 47USP47Ser876EAILEESTEKLKS2.8120.994
Ubiquitin carboxyl-terminal hydrolase 5USP5Ser760GRSAADSISESVP6.2331.933
DNA excision repair protein ERCC-6-likePICHSer810GTGSADSIATLPK5.6020.902
DNA excision repair protein ERCC-6-likePICHSer1000HSKTCLSWEFSEK1.8620.942
DNA excision repair protein ERCC-6-likePICHSer1004CLSWEFSEKDDEP2.0620.962
ATP-dependent DNA helicase 2 subunit 1Ku70Ser6MSGWESYYKTEG7.3840.964
Chromodomain-helicase-DNA-binding protein 4CHD-4Ser1676EKVEEKSAIDLTP8.7030.954
Chromodomain-helicase-DNA-binding protein 8CHD-8Ser1544RVLDNFSDIVEGV2.1230.962
ATP-dependent helicase SMARCAD1SMARCAD1Ser146RRNDDISELEDLS2.0641.002
TuberinTSC2Ser1346SSQEEKSLHAEEL2.7541.014
Rapamycin-insensitive companion of mTORRictorSer1370ITMKANSFESRLT2.9140.994
Proline-rich AKT1 substrate 1PRAS40Ser267RPRLNTSDFQKLK4.8330.994
Eukaryotic translation initiation factor 4E-binding protein 14E-BP1Ser112RAGGEESQFEMDI2.9830.973
Epidermal growth factor receptorEGFRSer1064CPIKEDSFLQRYS5.6521.024
Epidermal growth factor receptor substrate 15EPS15Ser467TAELEESVESGKA2.8721.022
Epidermal growth factor receptor substrate 15-like 1EPS15RSer708LDPFESSDPFSSS5.6820.953
Synaptojanin-1SYNJ1Ser830LDLLNASFQDESK4.9431.043
Phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing α polypeptidePIK3C2ASer108KLLLDDSFETKKT15.3331.024
Poly [ADP-ribose] polymerase 4PARP4Ser1288EKLLDLSWTESCK7.0540.974
Poly [ADP-ribose] polymerase 4PARP4Thr768NENLQDTVEKICI2.6241.034
DNA (cytosine-5)-methyltransferase 1DNMT1Ser456IFDANESGFESYE10.1430.992
Poly(A)-specific ribonuclease PARNPARNSer557HYYRNNSFTAPST2.7041.014
  • a Short protein name according to PhosphoSitePlus database (http://www.phosphosite.org).

  • b Phosphorylation sites are highlighted in bold type, and residues in −2 and /or +1 positions are underlined if consistent with Plk1 substrate consensus motif (D/E/N)X(pS/pT)(F/L/I/Y/W/V/M) (25).

  • c Average ± 3-MB-PP1 ratio measured in Plk1as and Plk1wt cell experiments.

  • d Number of biological replicate experiments in which the phosphosite ratio was quantified.

  • e Reported in vivo Plk1 site (41).

  • f Reported in vivo Plk1 site (42).

  • g Reported in vivo Plk1 site (43).

  • h Reported in vivo Plk1 sites (25).

  • i Reported in vitro Plk1 site (44); phosphosite position according to entry in IPI database, corresponds to Ser397 of the UniProtKB entry NEDD1_HUMAN.

  • j Reported in vitro Plk1 sites (45).

  • k Reported in vivo site for Plk3 (32).