Table II Cα distances of crosslinked sites in ISWI and its relatives
ISWIChd1Sso1653Rad54
SitesDistance (Å)SitesDist. (Å)SitesDist. (Å)SitesDist. (Å)
12Exp.aModelb123MWYc121Z6Ac121Z3Ic
190209≤13.610.643845710.450552413.12182389.0
361632≤20.011.561590111.9669n/agn/a407n/agn/a
361555≤13.66.06158196.06698555.84076156.3
578120≤12.112.184236827.9d,e87843735.8e63814037.4e
578172≤14.313.284242029.0d,e87848842.7e63819637.9e
578573≤13.613.6842837≥5.5d8788738.06386336.8
578585≤13.613.3842849n/af87888514.8e63864516.2e
578589≤17.016.8842858≥13.6d87888818.2e63864816.0
578644≤12.012.084291421.5d,e878n/agn/a638n/agn/a
578645≤14.614.384291524.6d,e878n/agn/a638n/agn/a
  • a Upper limits derived from crosslinking experiments. See Methods.

  • b Distances from model in Fig. 6B.

  • c PDB codes.

  • d A842 is missing from the structure precluding exact measurements. Lower limits were calculated from the distances to Y841 minus the maximal distance between neighboring Cα atoms (3.9Å).

  • e Bold face values exceed the distance limits measured for ISWI.

  • f Not available. Target amino acid is not present in the crystal structure.

  • g Not available. No sequence homology exists between ISWI and Sso1653/Rad54 in that region.