Table I F. tularensis strains and plasmids used in this study
Strain/plasmidCharacteristics/descriptionReference/source
Strain
    FSC200F. tularensis subsp. holarctica FSC200Ref. 47
    FSC679 (FSC200/ΔpglA)FSC200/in-frame deletion of codons 10–469 of FTT_0905Ref. 14
    FSC699 (FCS200/Δ1103)FSC200/in-frame deletion of codons 4–369 of FTT_1103Ref. 15
    FSC200/ΔwbtDEF:CmFSC200 with inactivated production of O-antigenThis study
    E. coli S17-1thi thr leu tonA supE recA::RP4–2-Tc::Mu Kn::Tn7Ref. 20
Plasmid
    pSMP22-RT1Suicide plasmid used to inactivate O-antigen cluster of F. tularensis subsp. tularensisRef. 19