Table II Proteome profile of high abundance protein sera fractions in human patients with cardiomyopathy of chagasic and other etiologies

The high abundance proteins in human sera samples from normal healthy subjects and chagasic and other cardiomyopathy patients were separated from low abundance proteins using IgY-12 high capacity LC10 proteome partitioning chromatography and resolved by a 2D-GE approach. The gel images were analyzed on Progenesis SameSpotstTM software, and normalized spot volumes were used for comparison of the two groups. Proteins spots with >2-fold change in chagasic sera were subjected to MALDI-TOF MS/MS analysis. The putative molecular/biological function and cellular location were identified using ingenuity pathway analysis and UniProt software. NA, no match available in public information databases.

Spot No.Gene nameProtein nameSwiss-Prot No.Molecular mass (kDa)Protein scorepIMolecular/biological functionCellular location
256, 257, 297, 301, 332a, 344, 374a, 396, 397, 400, 401, 445, 486, 487, 488, 505, 572, 614aALBAlbumin11357669,321.599–1005.92Transport, AntioxidativeExtracellular
297MTHFD1C-1-Tetrahydrofolate synthase115206101,495.381.276.89OxidoreductaseMitochondria
332CNOT10CCR4-NOT transcript ion complex subunit 107473398282,257.575.877.95Protein binding, mRNA metabolismCytosol
487C1RComplement C1r21851195680,066.892.195.82Peptidase/immune responseExtracellular
297, 505FGGFibrinogen γ2017828051,478.91005.37Protein binding/Signal transductionExtracellular
505HSDL2Hydroxysteroid dehydrogenase-like 27474952145,365.570.328.07OxidoreductaseMitochondrion
257, 438, 442, 500, 505, 528, 582, 600, 612IGHG1Ig γ1 chain C12103936,083.292.37–1008.46Antigen binding/immune responseExtracellular
442a, 500, 505, 528, 582, 600, 612IGHG2Ig γ2 chain C21851207935,877.876.96–1007.66-do--do-
438, 442a, 500, 505, 528, 582, 600, 612IGHG3Ig γ3 chain C19380636141,260.481.27–1008.23-do--do-
374aIGKCIg κ chain C region12514511,601.799.915.58-do--do-
517, 528, 612KRT1Keratin, cytoskeletal 123805440665,9991008.15-do--do-
528KRT2Keratin, cytoskeletal 223993865065,393.21008.07-do--do-
486, 612KRT9Keratin, cytoskeletal 923993888662,026.899.895.14-do--do-
517, 528KRT10Keratin, cytoskeletal 1026984976958,791.71005.13Protein binding/epidermisIntermediate fill
257LRRK2Leucine-rich repeat kinase 2120892285,873.989.476.34ATP binding/oxidative stress responseMitochondria
505MSH4MutS homolog 430316344104,689.274.157.16DNA repairNucleus
445, 517NRAPNebulin-related anchoring protein115502505196,950.382.929.24Ribosomal biogenesisNucleus
517PVALBParvalbumin α13110012,05199.384.98Ca2+ bindingCytoplasm
297PCID2PCI domain-containing protein 28568103445,999.899.188.78Protein binding/apoptosis inhibition
438PRODHProline dehydrogenase11936463959,192.877.486.43OxidoreductaseMitochondria
156LOC442122Uncharacterized protein7471096815,487.590.177.41Immune responseNA
156a, 301TFSerotransferrin701876,999.61006.81TransportExtracellular
528UTRNUtrophin215274104394,220.273.555.2Actinin binding/muscle developmentNucleus
374aZNF615Zinc finger protein 61516178434883,684.271.659.35DNA binding/TranscriptionNucleus
396ZNF528Zinc finger protein LOC40071317204615566,719.270.999.45-do--do-
505ZSWIN2Zinc finger SWIM domain-protein 215111272,673.984.428.93Zn2+ binding/apoptosisNA
  • a Selected, differentially expressed proteins that were recognized by Western blotting with anti-DNPH antibody and consisted DNPH-derivatized carbonyl adducts.