Table I Peptides identified in the DVEs of BC, BF, and BJ analyzed in the absence of proteinase inhibitors by ESI-Q-TOF or MALDI-Q-TOF and de novo sequencing from MS/MS spectra

*Peptides identified and sequenced from the LC-MS/MS analyses; **new LAAO fragments and new BPPs and pHpGs.

VenomHPLC fractionm/zzMass (Da)SequenceProtein or peptidePeptide name
BC6647.921293.8(b ion: 217.1)VG[Q/K]SAG[Q/K][I/L]YRLAAO**-
BC9592.421183.7<ENWPHPPMPPBPP**BPP-10d
BC10–11676.421350.7<ENWPHPPMPPAPBPP**BPP-12d
BC11–13839.41838.4<ENWPSP[Q/K]BPP**BPP-7b
BC12–14861.41860.4<ENAHPSP[Q/K]BPP**BPP-8a
BC151296.711295.5<EARPRPGP[Q/K][I/L]PPBPP**BPP-12e
BC16–20566.821131.5<ENWPSP[Q/K]VPPBPP**BPP-10e
BC16–20577.821153.6<ENAHPSP[Q/K]VPPBPP**BPP-11f
BC19–201370.711369.7<EGGWPRPGPE[I/L]PPBPPBPP-13a
BC*468.72935.4TTD[I/L]PFSRLAAO**-
BC*624.821247.6[I/L]VGGMD[Q/K][I/L]PTSMLAAO**-
BC*503.331506.9HHDHHAAVGGGGGGGGGApHpG**Bot.cf pHpG-1
BC*617.421232.7(b ion: 253.2)HAAVGGGGGGGGGApHpG**-
BC*516.321030.7[I/L]TEPVV[I/L]NFLAAO**-
BC*862.154305.4[I/L]TEPVV[I/L]NFFAGEYTA[Q/K]AHGW [I/L]DST[I/L][Q/K]SRDAARDVNRASLAAO**-
BF3503.231506.6HHDHHAAVGGGGGGGGGApHpG**Bot.cf pHpG-1
BF3754.321506.7HHDHHAAVGGGGGGGGGApHpG**Bot.cf pHpG-1
BF9–12, 14592.321182.7<ENWPHPPMPPBPP**BPP-10d
BF12, 14–15676.421350.7<ENWPHPPMPPAPBPP**BPP-12d
BF14881.51880.5<ERWPSP[Q/K]BPP**BPP-7c
BF19–23587.821173.6<ERWPSP[Q/K]VPPBPP**BPP-10f
BF191357.511356.5<EARPPHPP[I/L]PPAPBPPBPP-AP
BF24682.442725.4TA[Q/K]AHGW[I/L]DST[I/L][Q/K]SG[I/L] TA(y ion: 888.5)LAAO**-
BF27640.41278.6<EARPRHP[Q/K][I/L]PPBPP**BPP-11g
BF*664.321326.7VTVTY[Q/K]TSA[Q/K]ETLAAO**-
BF*852.421702.8TTFTPY[Q/K]F[Q/K]HFSEALAAO**-
BJ17–231189.711188.7<EARPPHPPIPPBPPBPP-11e
BJ17–23595.321188.5<EARPPHPPIPPBPPBPP-11e
BJ19–21672.321342.6<EGRPPHPP[I/L]PPAPBPP**BPP-13c
BJ19–261095.611094.6<EGRPPGPPIPPBPPBPP-11d
BJ19–26548.321094.6<EGRPPGPPIPPBPPBPP-11d
BJ20–23679.321356.7<EARPPHPPIPPAPBPPBPP-AP
BJ21–27648.821295.6<EARPRPGP[Q/K][I/L]PPBPP**BPP-12e
BJ21–27649.321296.7<EGGLPRPGPEIPPBPPBPP-13b
BJ23–27640.421278.7<EARPRHP[Q/K][I/L]PPBPP**BPP-11g
BJ27–291370.711369.7<EGGWPRPGPEIPPBPPBPP-13a
BJ27–29685.821369.6<EGGWPRPGPEIPPBPPBPP-13a
BJ27–311215.611214.6<ENWPRPQIPPBPPBPP-10b
BJ28–311299.711298.7<EWPRPTPQIPPBPPBPP-11a
BJ28–31650.321298.6<EWPRPTPQIPPBPPBPP-11a
BJ291281.711280.7<EWGRPPGPPIPPBPPBPP-12b
BJ29–321101.611100.6<EWPRPQIPPBPPBPP-9a
BJ29–32551.321100.6<EWPRPQIPPBPPBPP-9a
BJ321486.811485.8<EWAQWPRPQIPPBPPBPP-12c
BJ321684.911683.9<EWAQWPRPTPQIPPBPPBPP-14a
BJ*894.021785.9(b ion: 1099.6)PP[I/L]PPAPBPP**BPP
BJ*900.921799.9(b ion: 1113.4)PP[I/L]PPAPBPP**BPP
BJ*598.821195.6<ENWPHPQIPPBPPBPP-10c
BJ*575.321148.7<EGRHPP[I/L]PPAPBPP**BPP-11h
BJ*564.821127.7<ENGPRP[I/L]G[I/L]PPBPP**BPP-11i
BJ*603.821205.6<ENRHPP[I/L]PPAPBPP**BPP-11j
BJ*659.421316.7<EGRAPHPP[I/L]PPAPBPP**BPP-13d
BJ*380.541517.8AN[I/L]GPMR[I/L]PE[Q/K]HRLAAO**-
BJ*560.731679.0[I/L]E[I/L]A[Q/K]NG[I/L]SATSNP[Q/K]HLAAO**-
BJ*522.231563.7HHDHHAAVGGGGGGGGGGApHpG**Bot.ja pHpG-1