Table I Peptides and instrument parameters used in this study

c - carbamidomethylcysteine (+ C2H3NO), r - heavy labeled arginine (13C615N4), k - heavy labeled lysine (13C615N2).

#PeptideaPRM parametersSRM parameters
Mono. massParent m/zSched. RT (min)Parent m/zTransition m/zCE (%)Sched. RT (min)
1GVSAFSTWEk1118.5488560.281728.0–33.0560.584658.6462635.0–89.0
805.821
963.977
2HFLTLAPIk1046.6368524.325726.5–31.0
3ARPAcVDAr1024.5112513.26530.0–10.0
4SGWTcTQPGGr1281.5753608.773813.0–20.0
5EGQLAAGTcEIVTLDr1741.8544871.934529.0–36.0872.4321174.2453037.5–89.0
1245.323
1316.402
6LWSLAEIATSDLk1476.5364727.902740.0–53.0
7SEDEDEDEGDATr1648.8118739.27557.0–14.0
8DIQFGSQIk1042.5539522.284223.0–29.0522.557540.5552529.0–37.5
687.731
815.861
9HTGTPLGDIPYGk1436.6875682.358422.5–27.0
10NSWGTDWGEk1215.5330594.264025.5–30.5594.580743.7082729.0–37.5
800.760
986.971
11FSDLTEEEFr1362.7023641.794927.0–31.5642.134719.6752829.0–89.0
820.762
933.920
12SFEGTDYGk1010.4436506.229113.0–20.0506.491591.5562522.0–29.0
648.608
777.723
13TLNGIQLAr994.5799498.297221.0–26.5498.544610.6632622.0–35.0
667.715
781.818
14AFSQNSVLIk1113.6274557.821022.5–27.0
15WPGYLNGGr1028.5067515.260626.5–31.0515.532689.6772529.0–89.0
746.729
843.845
16GALDGEAPr894.4435448.229010.0–20.0448.441539.498231.0–29.0
654.586
767.745
17AETLVQAr896.4955449.255010.0–20.0449.471483.478231.0–29.0
596.636
697.741
18FLNPEWk940.4898471.252226.5–31.0
19LEQNPEESQDIk1453.7908719.351010.0–20.0
20SEASSSPPVVTSSSHSr1186.5135571.275610.0–15.0571.569583.511261.0–22.0
771.693
870.825
21APcQAGDLr996.4686499.241610.0–15.0499.520541.514241.0–22.0
669.644
829.839
22TWFQNQr988.4754495.245020.0–26.0
23TVFSSTQLcVLNDr1710.8048825.413231.0–40.0825.891899.9732937.5–89.0
1129.207
1216.285
24FSEVSADk889.4273445.720910.0–17.0445.944527.513221.0–29.0
656.628
746.706
25GLYEGTGr861.4220431.718310.0–20.0
  • a All peptides have a charge state (z) of +2, except for peptide #20 (z = +3).