Table I Summary of identified product ions from top-down MS of IgGs
Light chainHeavy chainTotal sequence coverage
N-Term. product ionsC-Term. product ionsNumber of unique cleavagesChain sequence coverageN-Term. product ionsC-Term. product ionsNumber of unique cleavagesChain sequence coverage
Orbitrap MS
    ETD, 360 scans, narrow isolation windowa46547233.8%48569020%24.4%
Orbitrap MS
    ETD, 1000 scans, wide isolation windowa45436329.6%577111726%27.1%
Orbitrap MS
    ETD, combinedb54679243.2%608512527.8%32.7%
qTOF MS
    ETDc21153114.4%11627316.1%15.4%
Orbitrap MS
    CIDd24325324.8%2620429.5%14.5%
  • a Measurements performed in the present study on intact human IgG1, kappa, with c-ions considered for the N-terminus and z- and y-ions considered for the C-terminus.

  • b Values calculated by combining the backbone cleavage sites of the two Orbitrap FTMS experiments.

  • c Measurements performed in a previous work (Ref. 25) on an intact IgG1, kappa, with c-ions considered for the N-terminus and z-ions considered for the C-terminus.

  • d Data derived from Ref. (29); measurements performed on reduced and alkylated human IgG2, with b-ions considered for the N-terminus and y-ions considered for the C-terminus.