Table I Summary of the results; identified proteins for which the quantitation data passed the quality thresholds are listed in descending order of T/C value
Entry nameaMassL/H (Ctl)bL/H (+Trn)cT/CdBead haloeNLSh (reference)
1RA1L3_HUMAN343730.0510.3376.602(pseudogene)i
2ROA2_HUMAN374640.0400.2446.153++PYj (30)
3ROA1_HUMAN388370.0860.4234.910++PY (30)
4HNRPD_HUMAN385810.1330.4133.104+PY (37)
5ROAA_HUMAN363160.1430.4142.901++PYj (this study)
6RL3_HUMAN463650.0980.2292.339++(++)BIB (this study)
7HNRH1_HUMAN494840.3340.6151.839++PY (29)
8CPSF6_HUMAN593440.2430.3281.349Degr.fPY (14, 24)
9SF3B2_HUMAN1002790.4920.5781.176Degr.f(+)BIB (this study)
10RU2A_HUMAN285120.7140.8061.129++(+)BIB (this study)
11RL27_HUMAN157880.1200.1341.121++(++)BIB (this study)
12SNRPA_HUMAN312590.1220.1351.111+PYj (24)
13SF3B3_HUMAN1365750.6980.7521.077Not exp.g
14SMC1A_HUMAN1437710.1320.1371.039Not exp.g
15PARP1_HUMAN1138110.1490.1511.013Degr.f
16IMB1_HUMAN984200.0410.0411.001
17RS12_HUMAN149050.2960.2890.976+
18HNRDL_HUMAN465800.1770.1680.946++PY (38)
19WBP11_HUMAN699540.1970.1830.928
20RL7_HUMAN292640.1050.0970.928
21PPIB_HUMAN237850.1140.1040.914±
22RL26_HUMAN172480.1180.1070.907
23RL18A_HUMAN210340.1290.1170.905
24SK2L2_HUMAN1187560.1350.1220.903
25ERP29_HUMAN290320.0860.0780.903
26NHP2_HUMAN175320.1040.0930.889
27RL12_HUMAN179790.1360.1180.868
28GRP78_HUMAN724020.2430.2110.868
29RAB7A_HUMAN237600.8790.7590.863
30PDIA3_HUMAN571460.1090.0940.861
31HNRPU_HUMAN912690.0710.0610.860+
32RL10A_HUMAN249870.1380.1180.855
33PRKDC_HUMAN4737490.1810.1540.853
34RL14_HUMAN235310.1420.1210.849
35RL8_HUMAN282350.1270.1080.848
36RS4X_HUMAN298070.2840.2400.844
37RLA0L_HUMAN345140.1950.1630.834
38NPM_HUMAN327260.0810.0680.832
39RL7A_HUMAN301480.1300.1070.822
40RL11_HUMAN204680.1470.1190.812
41RS11_HUMAN185900.3110.2510.808
42RL13A_HUMAN236190.1290.1040.808
43RL19_HUMAN235650.1270.1020.801
44RBBP7_HUMAN481320.9590.7680.800
45PDIA6_HUMAN484900.0780.0610.781
46RS10_HUMAN188860.3060.2380.777
47RS25_HUMAN137910.3340.2580.771
48RS29_HUMAN69000.2950.2260.767
49CPSM_HUMAN1659750.0470.0360.764
50RS23_HUMAN159690.2720.2070.763
51RS13_HUMAN172120.3040.2320.763
52RS8_HUMAN244750.2860.2160.756
53DJB11_HUMAN407740.0990.0730.742
54RL24_HUMAN178820.1490.1100.739
55RS7_HUMAN221130.3470.2490.719
56PAIRB_HUMAN449950.3420.2450.717
57RS18_HUMAN177080.3280.2350.717
58MCM3_HUMAN915510.3500.2500.714
59RS9_HUMAN226350.2810.1980.705
60RL5_HUMAN345690.2530.1770.698(39)k
61GBLP_HUMAN355110.3510.2450.697
62RS3_HUMAN268420.3470.2410.695
63PCNA_HUMAN290920.7560.5240.694
64RS2_HUMAN315900.3700.2550.689(40)
65IF2A_HUMAN363740.9120.6280.689
66HNRPQ_HUMAN697880.7290.4980.683
67RL4_HUMAN479530.1430.0980.682
68ENPL_HUMAN926960.1040.0700.675
69HNRPK_HUMAN512300.5970.3990.668
70XRCC6_HUMAN700840.4050.2690.666
71APEX1_HUMAN359310.9480.6310.666
72NSUN2_HUMAN872140.4030.2660.661
73RS3A_HUMAN301540.2940.1940.660
74ALDR_HUMAN362300.3230.2130.659
75DHX15_HUMAN916730.5550.3640.656
76RL18_HUMAN217350.1160.0750.647
77BTF3_HUMAN222110.8510.5480.644
78HMCS1_HUMAN578280.2660.1690.637
79CBX3_HUMAN209690.1890.1200.632
80PSME3_HUMAN296020.9040.5710.631
81MCM2_HUMAN1025160.5770.3630.628
82ACTN4_HUMAN1052450.4890.2990.612
83PRP19_HUMAN556030.2740.1670.610
84PABP1_HUMAN708540.6440.3700.575
85DNMT1_HUMAN1853880.5560.3150.566
86NUCL_HUMAN766250.1390.0760.549
87MCM6_HUMAN938010.5900.3200.542
88SERA_HUMAN573560.5470.2960.541
89VINC_HUMAN1242920.6440.3280.509
90DEK_HUMAN429330.1250.0520.415
  • a UniProtKB/Swiss-Prot entry name.

  • b Light/heavy ratio of the control reaction.

  • c Light/heavy ratio of the +Trn reaction.

  • d The light/heavy ratio of the +Trn reaction was divided by the light/heavy ratio of the control reaction.

  • e Results of the bead halo assay: ++ and +, positive; −, negative; ±, unclear; blank, not done; (++) and (+), positive for Trn binding when GFP-Lys/Arg-rich segment fusions were assayed (Fig. 3D).

  • f,g The assays were not done or the results were obscure, because the proteins were not expressed (g) or degraded extensively (f) in E. coli.

  • h Potential NLS for Trn-binding: PY, PY-NLS; BIB, BIB-domain-like NLS; –, undetermined; blank, not examined.

  • i Three peptides were matched in the Mascot search, but their sequences exist in ROA1 also.

  • j Predicted in this study or the indicated reference.

  • k Result of Xenopus L5.