Table I Tandem mass spectrometric identification of O-linked HexNAc-modified peptides isolated from differentiating osteoblasts

Novel sites of modification are in boldface, and ambiguous sites of modification are underlined. An asterisk indicates a site of HexNAc modification either at or proximal to a known site of phosphorylation (± four amino acids) (see supplemental Table 2).

AccessionProteinGene symbolPeptideSiteMS/MSzm/z
P45481CREB-binding proteinCBP135QAASTSGPTPPAS(HexNAc)QALNPQAQK156Ser-147CID21177.59
2344SPAPVQSPRPQSQPPHS(HexNAc)SPSPR2365Ser-2360*ETD4632.51
Q8BT14CCR4-NOT transcription complex subunit 4CNOT4309SNPVIPIS(HexNAc)SSNHSAR323Ser-316ETD3591.11
Q61191Host cell factor 1HCF1612TAAAQVGTSVSSAANTSTRPIITVHK637620–623*ETD4745.12
1232QPETYHT(HexNAc)YTTNTPTTTR1248Thr-1238ETD3739.96
1232QPETYHTYTT(HexNAc)NTPTTTR1248Thr-1241*ETD3739.95
P48678Prelamin A/CLMNA600AAGGAGAQVGGSISSGSSASSVTVTR625613–624CID21213.58
Q8BFW7Lipoma-preferred homologLPP179SATKPQPAPQAAPIPVTPIGTLKPQPQPVPAS (HexNAc)YT TASTSSRPTFNVQVK227Ser-210ETD51053.02
Q811L6Microtubule- associated serine/threonine-protein kinase 4MAST42172SATATTTAIATTTTTTSAGHSDCSSHK21982176–2177ETD4719.17
Q02780Nuclear factor 1 A-typeNFI-A352SGFSSPSPSQTSSLGTAFTQHHRPVITGPR381362–363*ETD5667.41
Q6PIJ4Nuclear factor related to κB-binding proteinNFRκB1258IQTVPASHLQQGT(HexNAc)ASGSSK1276Thr-1270ETD3701.34
Q02819Nucleobindin 1NUCB1399KQQLQEQS(HexNAc)APPSKPDGQLQFR419Ser-406ETD4655.05
P81117Nucleobindin 2NUCB2400KLQQGIAPS(HexNAc)GPAGELK415Ser-408ETD3599.92
Q80U93Nuclear pore complex proteinNUP214489SSASVTGEPPLYPTGS(HexNAc)DSSR508Ser-504*CID21100.67
Q6ZPK0PHD finger protein 21APHF21A278FTPTTLPTS(HexNAc)QNSIHPVR294Ser-286ETD3701.00
Q9DBG5Perilipin 3PLN369TLTTAAVSTAQPILSK8476–77CID2903.71
Q6NXI6Regulation of nuclear pre-mRNA domain containing protein 2RPRD2408EKPVEKPAVS(HexNAc)T(HexNAc)GVPTK423Ser-417, Thr-418ETD3692.22
Q02614SAP30-binding proteinS30BP229KGTTT(HexNAc)NATATSTSTASTAVADAQK252Thr-233ETD3829.74
Q689Z5Protein strawberry notch homolog 1SBNO1106TPPAT(HexNAc)T(HexNAc)NRQTITLTK120Thr-110, Thr-111ETD3684.62
Q8CF89TGF-β-activated kinase 1/MAP3K7- binding protein 1TAB1385VYPVSVPYSSAQSTSK400393–399CID2953.05
Q99K90TGF-β-activated kinase 1/MAP3K7-binding protein 2TAB2453VVVT(HexNAc)QPNTKYTFK465Thr-456ETD3576.97
Q80X50Ubiquitin-associated protein 2-likeUBAP2l621RYPSS(HexNAc)ISSSPQKDLTQAK638Ser-625*ETD3733.73
P83741Serine/threonine-protein kinase WNK1WNK11834DGTEGHVTATS(HexNAc)SGAGVVK18511,844ETD3625.97