Table I Significantly modulated proteins in gastrocnemius muscle of 3-week-old dystrophin deficient mdx mice relative to C57BL/6 mice
AccessionaProtein namebFold changec
P20152Vimentin3.51
P07356Annexin A23.18
P31001Desmin2.69
Q6ZWV360S ribosomal protein L102.46
P3597960S ribosomal protein L122.25
P1925360S ribosomal protein L13a2.18
P6224240S ribosomal protein S82.09
P3598060S ribosomal protein L182.03
P6290840S ribosomal protein S32.02
Q6ZWN540S ribosomal protein S91.99
Q9CXW460S ribosomal protein L111.99
P6798460S ribosomal protein L221.96
P1413140S ribosomal protein S161.95
P68040Guanine nucleotide-binding protein subunit beta-2-like 11.94
Q8BTM8Filamin-A1.88
O88990Alpha-actinin-31.79
Q9JI91Alpha-actinin-21.79
P26039Talin-11.77
P15864Histone H1.21.77
Q00897Alpha-1-antitrypsin 1–41.74
Q921I1Serotransferrin1.71
P62962Profilin-11.65
P07724Serum albumin1.64
P97457Myosin regulatory light chain 2, skeletal muscle isoform1.64
P01027Complement C31.61
P14824Annexin A61.55
P99024Tubulin beta-5 chain1.52
P68372Tubulin beta-2C chain1.47
P2002978 kDa glucose-regulated protein1.46
Q9ERD7Tubulin beta-3 chain1.43
Q9JK37Myozenin-11.42
Q8VHX6Filamin-C1.42
P48036Annexin A51.39
Q99PT1Rho GDP-dissociation inhibitor 11.38
P23953Liver carboxylesterase N1.36
Q62234Myomesin-11.29
P6310114–3-3 protein zeta/delta1.28
Q9CQZ5NADH dehydrogenase (ubiquinone) 1α subcomplex subunit 61.25
Q3V1D3AMP deaminase 11.25
Q8QZY1Eukaryotic translation initiation factor 3 subunit L1.23
Q9ERS2NADH dehydrogenase (ubiquinone) 1α subcomplex subunit 131.22
Q9WUB3Glycogen phosphorylase, muscle form1.21
P20108Thioredoxin-dependent peroxide reductase, mitochondrial1.18
P99029Peroxiredoxin-5, mitochondrial1.17
Q64727Vinculin1.17
Q9D051Pyruvate dehydrogenase E1 component subunit β mitochondrial1.07
Q9D6R2Isocitrate dehydrogenase (NAD) subunit α, mitochondrial−1.12
Q99KI0Aconitate hydratase, mitochondrial−1.12
P47934Carnitine O-acetyltransferase−1.15
P48962ADP/ATP translocase 1−1.17
Q9WUZ7SH3 domain-binding glutamic acid-rich protein−1.17
O55126Protein NipSnap homolog 2−1.18
P32848Parvalbumin alpha−1.18
P41216Long-chain-fatty-acid–CoA ligase 1−1.19
Q61425Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial−1.21
Q6P8J7Creatine kinase, sarcomeric mitochondrial−1.23
Q99JY0Trifunctional enzyme subunit beta, mitochondrial−1.23
P51174Long-chain specific acyl-CoA dehydrogenase, mitochondrial−1.24
Q8BMS1Trifunctional enzyme subunit alpha, mitochondrial−1.25
P421253,2-trans-enoyl-CoA isomerase, mitochondrial−1.27
P05201Aspartate aminotransferase, cytoplasmic−1.29
P13412Troponin I, fast skeletal muscle−1.35
P16125l-lactate dehydrogenase B chain−1.36
P11404Fatty acid-binding protein, heart−1.38
A2ASS6Titin−1.43
P68033Actin, alpha cardiac muscle 1−1.64
P68134Actin, alpha skeletal muscle−1.65
P13541Myosin-3−1.71
P13542Myosin-8−1.83
Q5SX40Myosin-1−1.86
Q5SX39Myosin-4−2.12
P58771Tropomyosin alpha-1 chain−2.33
P58774Tropomyosin beta chain−2.63
  • a Accession numbers are from the Uniprot database.

  • b Protein names are from the Uniprot database; significantly different protein modulations (p < 0.05) were determined using the non-parametric Wilcoxson rank sum test.

  • c Fold changes were calculated using mdx/wt (unlabeled/labeled) ratios quantitated using Integrated Proteomics Pipeline software. Ratios were obtained from n = 3 mice/group.